eIF2 alpha/EIF2S1 293T Cell Transient Overexpression Lysate

Product Discontinued

eIF2 alpha/EIF2S1 293T Cell Transient Overexpression Lysate Summary

• Description: Quality control test: Transient overexpression cell lysate was tested with Anti-EIF2S1 antibody by Western Blots. Plasmid: pCMV-EIF2S1 full-length
• Specificity: eIF2 alpha/EIF2S1 293T Cell Transient Overexpression Lysate(Denatured)
• Gene: EIF2S1

Applications/Dilutions

• Dilutions:
  • Western Blot
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

Lysate Details for Array

• Type: 293T Cell Transient Overexpression
• Protein State: Denatured

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for eIF2 alpha/EIF2S1 293T Cell Transient Overexpression Lysate

• eIF2 alpha
• EIF2
• EIF-2
• EIF2A
• EIF-2A
• EIF-2alpha
• EIF2S1 eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
• EIF2S1

Background

EIF2S1, also known as eIF2a, is the alpha subunit of the translation initiation factor eIF2 complex which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. The phosphorylation state of eIF2S1 controls the rate of tRNA translation. When eIF2a is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2S1 is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation. Recombinant human EIF2S1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: EIF2S1