eIF2 alpha/EIF2S1 Antibody (430516) [Alexa Fluor® 750]

• Reactivity: Hu
• Applications: WB
• Clone: 430516
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 750

eIF2 alpha/EIF2S1 Antibody (430516) [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human eIF2α
  Met1-Asp315
  Accession # Q53XC0
• Specificity: Detects human eIF2 alpha in Western blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for eIF2 alpha/EIF2S1 Antibody (430516) [Alexa Fluor® 750]

• eIF2 alpha
• EIF2
• EIF-2
• EIF2A
• EIF-2A
• EIF-2alpha
• EIF2S1 eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
• EIF2S1

Background

Eukaryotic translation initiation factor 2 alpha subunit (eIF2 alpha ) is a subunit of the eIF2 protein, an important regulator of translation initiation. Phosphorylation of eIF2 alpha on Ser 52 increases the affinity of eIF2 alpha for eIF2B, a guanine nucleotide exchange factor needed for the recycling of eIF2-GDP to eIF2-GTP. Reduction of eIF2-GTP levels leads to the suppression of the overall rate of protein synthesis. Heme-regulated inhibitor (HRI), ER-resident protein kinase PERK, dsRNA activated protein kinase PKR, and the homologue of the S. cerevisiae protein kinase GCN2 are all eIF2 alpha kinases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.