EGR2 Antibody [DyLight 405]

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Product Details

Summary
Reactivity Hu, Mu, PoSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 405

EGR2 Antibody [DyLight 405] Summary

Immunogen
A synthetic peptide made to a portion of human EGR2 (within residues 200-300). [Swiss-Prot# P11161]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EGR2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
This EGR2 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~50 kDa. There is also a strong non-specific band at ~75 kDa. In ICC/IF nuclear staining was observed in Hela cells.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, mouse (review data) and porcine. Immunogen sequence has 86% homology to mouse, rat, chicken and Xenopus proteins.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for EGR2 Antibody [DyLight 405]

  • AT591
  • CMT1D
  • CMT4E
  • early growth response 2 (Krox-20 homolog, Drosophila)
  • early growth response 2
  • early growth response protein 2
  • EGR-2
  • FLJ14547
  • Krox-20 homolog, Drosophila
  • KROX-20, Drosophila, homolog (early growth response-2)
  • KROX20DKFZp686J1957
  • Zinc finger protein Krox-20

Background

EGR2 (E3 SUMO-protein ligase EGR2) protein is a member of EGR C2H2-type zinc-finger family and is a sequence-specific DNA-binding transcription factor that binds to two specific DNA sites located in HOXA4 promotor. EGR2 is an E3 SUMO-protein ligase that facilitates SUMO1 conjugation to its coregulators NAB1/NAB2, whose sumoylation down-regulates EGR2's own transcriptional activity. EGR2 interacts with HCFC1, UBC9 and WWP2, and WWP2 mediated ubiquitinization of EGR2 results in its proteasomal degradation. EGR2 protein activates several genes which are implicated in the formation and maintenance of myelin, the fatty substance that covers and protects nerve cells. EGR2 is considered a "master" transcription factor that regulates PNS myelination and plays a key role in myelin homeostasis. Besided peripheral nerve myelination, EGR2 plays important role in T cell maturation, hindbrain segmentation, and lipid biosynthesis. Genetic alteration of EGR2 results in a variety of relatively severe demyelinating neuropathies such as congenital hypomyelinating neuropathy (CHN), childhood-onset Dejerine-Sottas neuropathy (DSN), and Charcot-Marie-Tooth disease type 1D (CMT1D).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EGR2