EGR1 Antibody (406913)

EGR1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 15 µg/mL Rat Anti-Human EGR1 Monoclonal Antibody (Catalog # MAB2818) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

EGR1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 15 µg/mL Rat Anti-Human EGR1 Monoclonal Antibody (Catalog # MAB2818) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 406913
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

EGR1 Antibody (406913) Summary

• Immunogen: E. coli-derived recombinant human EGR1
  Gln282-Ser433
  Accession # P18146
• Specificity: Detects human EGR1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: EGR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EGR1 Antibody (406913)

• AT225Transcription factor ETR103
• early growth response 1
• early growth response protein 1
• EGR1
• EGR-1
• G0S30
• KROX24
• KROX-24
• Nerve growth factor-induced protein A
• NGFIA
• NGFI-ATranscription factor Zif268
• TIS8
• ZIF-268
• Zinc finger protein Krox-24
• ZNF225
• ZNF225Zinc finger protein 225

Background

Early Growth Response 1, (EGR1) belongs to the EGR family of zinc finger transcription factors and regulates the expression of several tumor suppressors. Its expression is induced by diverse signals that initiate growth and differentiation. EGR1 suppresses or activates cell growth depending on the tumor type. Within the region that includes its three zinc finger domains, human EGR1 shares 99% aa sequence identity with mouse and rat EGR1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EGR1 Antibody (MAB2818)(2)

Publications using MAB2818

• Julia Terreros-Roncal, Miguel Flor-García, Elena P. Moreno-Jiménez, Noemí Pallas-Bazarra, Alberto Rábano, Nirnath Sah et al. Activity-Dependent Reconnection of Adult-Born Dentate Granule Cells in a Mouse Model of Frontotemporal Dementia The Journal of Neuroscience 2019-07-17 [PMID: 31133559] (Immunohistochemistry, Transgenic Mouse)
• X Zhang, Z Yang, Y Heng, C Miao MicroRNA?181 exerts an inhibitory role during renal fibrosis by targeting early growth response factor?1 and attenuating the expression of profibrotic markers Mol Med Rep, 2019-02-18;0(0):. 2/18/2019 [PMID: 30816527]

Bioinformatics

• Gene Symbol: EGR1
• Uniprot:
  • P18146()