EEF1G Antibody

Western Blot: EEF1G Antibody [NB100-2262] - Detection of Human and Mouse EEF1G on HeLa whole cell lystate using NB100-2262.

Immunohistochemistry: EEF1G Antibody [NB100-2262] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-eEF1G used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

EEF1G Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 200 and 250 of human Eukaryotic Translation Elongation Factor 1 Gamma using the numbering given in entry NP_001395.1 (GeneID 1937).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EEF1G
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30147651).
• Control:

  EEF1G Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for EEF1G Antibody

• eEF-1B gamma
• EF-1-gamma
• EF1Gelongation factor 1-gamma
• eukaryotic translation elongation factor 1 gamma
• GIG35
• pancreatic tumor-related protein
• PRO1608
• translation elongation factor eEF-1 gamma chain

Background

This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EEF1G Antibody (NB100-2262)(1)

Publication using NB100-2262

• Furutani Y, Yoshihara Y. Proteomic Analysis of Dendritic Filopodia-Rich Fraction Isolated by Telencephalin and Vitronectin Interaction Front Synaptic Neurosci 2018-08-10 [PMID: 30147651] (ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: EEF1G
• Entrez:
  • 1937(Human)
  • 67160(Mouse)
• Uniprot:
  • NP_001395.1(Human)