EEF1D Antibody

Images

 
Western Blot: EEF1D Antibody [NBP2-16273] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: EEF1D Antibody [NBP2-16273] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: EEF1D Antibody [NBP2-16273] - Immunohistochemical analysis of paraffin-embedded AdCA, using antibody at 1:250 dilution.
Western Blot: EEF1D Antibody [NBP2-16273] - EEF1D antibody detects EEF1D protein by Western blot analysis. A. 30 ug THP-1 whole cell lysate/extract B. 30 ug HL-60 whole cell lysate/extract C. 30 ug NCI-H929 whole cell ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

EEF1D Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human EEF1D. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EEF1D
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EEF1D Antibody

  • Antigen NY-CO-4
  • EF-1D
  • EF-1-delta
  • EF1DFP1047
  • elongation factor 1-delta
  • eukaryotic translation elongation factor 1 delta (guanine nucleotide exchangeprotein)
  • FLJ20897
  • guanine nucleotide exchange protein

Background

This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit functions as guanine nucleotide exchange factor. It is reported that this subunit interacts with HIV-1 Tat, and thus it represses the translation of host-cell, but not HIV-1, mRNAs. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EEF1D