EEF1D Antibody

Western Blot: EEF1D Antibody [NBP2-16273] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: EEF1D Antibody [NBP2-16273] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: EEF1D Antibody [NBP2-16273] - Immunohistochemical analysis of paraffin-embedded AdCA, using antibody at 1:250 dilution.

Western Blot: EEF1D Antibody [NBP2-16273] - EEF1D antibody detects EEF1D protein by Western blot analysis. A. 30 ug THP-1 whole cell lysate/extract B. 30 ug HL-60 whole cell lysate/extract C. 30 ug NCI-H929 whole cell lysate/extract10 % SDS-PAGEEEF1D antibody dilution: 1:1000.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

EEF1D Antibody Summary

• Description: Novus Biologicals Rabbit EEF1D Antibody (NBP2-16273) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human EEF1D. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EEF1D
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 71 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for EEF1D Antibody

• Antigen NY-CO-4
• EF-1D
• EF-1-delta
• EF1DFP1047
• elongation factor 1-delta
• eukaryotic translation elongation factor 1 delta (guanine nucleotide exchangeprotein)
• FLJ20897
• guanine nucleotide exchange protein

Background

This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit functions as guanine nucleotide exchange factor. It is reported that this subunit interacts with HIV-1 Tat, and thus it represses the translation of host-cell, but not HIV-1, mRNAs. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: EEF1D