EEF1D Antibody

Images

 
Western Blot: EEF1D Antibody [NBP1-32013] - Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: EEF1D Antibody [NBP1-32013] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.
Western Blot: EEF1D Antibody [NBP1-32013] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE, antibody diluted at 1:3000.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.91 mg/ml

Order Details

EEF1D Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human EEF1D. The exact sequence is proprietary.
Predicted Species
Rat (92%), Bovine (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EEF1D
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.91 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EEF1D Antibody

  • Antigen NY-CO-4
  • EF-1D
  • EF-1-delta
  • EF1DFP1047
  • elongation factor 1-delta
  • eukaryotic translation elongation factor 1 delta (guanine nucleotide exchangeprotein)
  • FLJ20897
  • guanine nucleotide exchange protein

Background

This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit functions as guanine nucleotide exchange factor. It is reported that this subunit interacts with HIV-1 Tat, and thus it represses the translation of host-cell, but not HIV-1, mRNAs. Several alternatively spliced transcript variants have been found for this gene, however, the full length nature of only two variants has been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EEF1D