ECM-1/Secretory Component P85 Antibody (SC05) - IHC-Prediluted

Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (SC05) - IHC-Prediluted [NBP3-05763] - IHC-Prediluted - Formalin-fixed, paraffin-embedded human Colon Carcinoma.

• Reactivity: Hu, Rt
• Applications: ICC/IF, IHC
• Clone: SC05
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: IHC-Prediluted

ECM-1/Secretory Component P85 Antibody (SC05) - IHC-Prediluted Summary

• Description: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
• Immunogen: Full length native protein (purified) corresponding to Human IgA ECM-1/Secretory Component P85. Affinity-purified secretory component from human colostrum. (Uniprot: Q16610 )
• Localization: Cell surface and cytoplasmic
• Specificity: This monoclonal antibody reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ECM1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1-2ug
  • Immunohistochemistry-Paraffin 1-2ug
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for ECM-1/Secretory Component P85 Antibody (SC05) - IHC-Prediluted

• ECM1
• extracellular matrix protein 1
• Free Secretory Component (FSC)
• p85
• pIgA-Secretory Component Complex
• pIgM-Secretory Component Complex
• Secretory Component (SC)
• Secretory Component Glycoprotein
• Secretory Component P85
• sIgA-Secretory Component Complex

Background

Examples of positive human tissues: secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ECM1