ECH1 Overexpression Lysate

Images

 
Western Blot: ECH1 Overexpression Lysate (Adult Normal) [NBL1-10098] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ECH1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ECH1 Overexpression Lysate Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC205622 and based on accession number NM_001398. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens enoyl Coenzyme A hydratase 1, peroxisomal (ECH1), mRNA.
Gene
ECH1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
35.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ECH1 Overexpression Lysate

  • delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
  • delta3,5-delta2,4-dienoyl-CoA isomerase
  • dienoyl-CoA isomerase
  • EC 5.3.3.-
  • enoyl CoA hydratase 1, peroxisomal
  • enoyl Coenzyme A hydratase 1, peroxisomal
  • HPXEL
  • peroxisomal enoyl-CoA hydratase 1

Background

This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

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Other Available Formats

NBL1-10098

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Bioinformatics

Gene Symbol ECH1