EBF-1 Antibody

Western Blot: EBF-1 Antibody [NBP1-28471] - Analysis of EBF1 in Human Adipose lysate (35ug protein in RIPA buffer) using this antibody at 0.3 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

EBF-1 Antibody Summary

• Description: Novus Biologicals Goat EBF-1 Antibody (NBP1-28471) is a polyclonal antibody validated for use in WB and ELISA. Anti-EBF-1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-HNQLPALANTSV corresponding to internal region according to NP_076870.1.
• Epitope: C terminus
• Specificity: This antibody is expected not to cross-react with EBF2, EBF3 and EBF4.
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: EBF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.3 - 0.1 ug/ml
• Application Notes: IP: An anonymous customer has reported successful from induced 3T3-L1 expressing FLAG-EBF1. WB: Approx. 75 kDa band observed in human adipose lysates (calculated MW of 64.5 kDa band according to NP_076870.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for EBF-1 Antibody

• COE1
• Collier, Olf and EBF transcription factor 1
• early B-cell factor 1
• Early B-cell factor
• EBF1
• EBF-1
• EBFFLJ41763
• O/E-1
• OE-1
• OLF1
• OLF1FLJ39389
• olfactory neuronal transcription factor 1
• transcription factor COE1

Background

Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3' (Bysimilarity)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EBF-1 Antibody (NBP1-28471)(1)

Publication using NBP1-28471

• Lagergren A, Mansson R, Zetterblad J et al. The CXCL12, periostin and CCL9 genes are direct targets for early B-cell factor (EBF) in OP-9 stroma cells. J Biol Chem 2007-03-20 [PMID: 17374609]

Bioinformatics

• Gene Symbol: EBF1
• Entrez:
  • 1879(Human)
  • 13591(Mouse)
  • 116543(Rat)
• Uniprot:
  • NP_076870.1(Human)