Western Blot: EBAG9/RCAS1 Antibody [NB100-41096] - Staining of A431 lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by ...read more
Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody [NB100-41096] - staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
WB: Approx. 26 kDa band observed in HEK293 and A431 lysates (calculated MW of 24.4 kDa band according to NP_004206.1and NP_004206.1). A minor band of unknown identity was also consistently observed at 75 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC: Paraffin embedded Human Skeletal Muscle. Use in ICC/IF was reported in scientific literature (PMID: 31433987).
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for EBAG9/RCAS1 Antibody
Cancer-associated surface antigen RCAS1
EB9cancer associated surface antigen
estrogen receptor binding site associated, antigen, 9
Estrogen receptor-binding fragment-associated gene 9 protein
receptor-binding cancer antigen expressed on SiSo cells
This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor which binds to the estrogen-responsive element (ERE) found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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