EBAG9/RCAS1 Antibody

Western Blot: EBAG9/RCAS1 Antibody [NB100-41096] - Staining of A431 lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence. (0.2ug/ml)

Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody [NB100-41096] - staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

EBAG9/RCAS1 Antibody Summary

• Immunogen: Peptide with sequence C-KEAQRLMKKEQN corresponding to C-Terminus according to NP_004206.1.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: EBAG9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 26 kDa band observed in HEK293 and A431 lysates (calculated MW of 24.4 kDa band according to NP_004206.1and NP_004206.1). A minor band of unknown identity was also consistently observed at 75 kDa. This band was successfully blocked by incubation with the immunizing peptide.
  IHC: Paraffin embedded Human Skeletal Muscle. Use in ICC/IF was reported in scientific literature (PMID: 31433987).
• Control:

  EBAG9/RCAS1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for EBAG9/RCAS1 Antibody

• Cancer-associated surface antigen RCAS1
• EB9cancer associated surface antigen
• estrogen receptor binding site associated, antigen, 9
• Estrogen receptor-binding fragment-associated gene 9 protein
• RCAS1PDAF
• receptor-binding cancer antigen expressed on SiSo cells

Background

This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor which binds to the estrogen-responsive element (ERE) found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EBAG9/RCAS1 Antibody (NB100-41096)(2)

Publications using NB100-41096

• Sola-Riera C, Gupta S, Maleki KT et al. Hantavirus Inhibits TRAIL-Mediated Killing of Infected Cells by Downregulating Death Receptor 5 Cell Rep 2019-08-20 [PMID: 31433987] (ICC/IF, Human)
• Ruder C, Reimer T, Delgado-Martinez I et al. EBAG9 adds a new layer of control on large dense-core vesicle exocytosis via interaction with Snapin. Mol Biol Cell 2005-03-01 [PMID: 15635093]

Bioinformatics

• Gene Symbol: EBAG9
• Entrez:
  • 9166(Human)
  • 55960(Mouse)
  • 299864(Rat)
• Uniprot:
  • NP_004206.1(Human)