EBAG9/RCAS1 Antibody

Images

 
Western Blot: EBAG9/RCAS1 Antibody [NB100-41096] - Staining of A431 lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by ...read more
Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody [NB100-41096] - staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

EBAG9/RCAS1 Antibody Summary

Immunogen
Peptide with sequence C-KEAQRLMKKEQN corresponding to C-Terminus according to NP_004206.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EBAG9
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 26 kDa band observed in HEK293 and A431 lysates (calculated MW of 24.4 kDa band according to NP_004206.1and NP_004206.1). A minor band of unknown identity was also consistently observed at 75 kDa. This band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Skeletal Muscle
Positive Control
EBAG9/RCAS1 Lysate (NBL1-10090)
Publications
Read Publication using NB100-41096.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EBAG9/RCAS1 Antibody

  • Cancer-associated surface antigen RCAS1
  • EB9cancer associated surface antigen
  • estrogen receptor binding site associated, antigen, 9
  • Estrogen receptor-binding fragment-associated gene 9 protein
  • RCAS1PDAF
  • receptor-binding cancer antigen expressed on SiSo cells

Background

This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor which binds to the estrogen-responsive element (ERE) found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EBAG9/RCAS1 Antibody (NB100-41096)(1)

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EBAG9
Entrez
Uniprot