EBAG9/RCAS1 Overexpression Lysate (Native)

Images

 
Western Blot: EBAG9/RCAS1 Overexpression Lysate (Adult Normal) [NBL1-10090] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EBAG9/RCAS1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

EBAG9/RCAS1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC205364 and based on accession number NM_004215. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens estrogen receptor binding site associated, antigen, 9 (EBAG9), transcript variant 1, mRNA.
Gene
EBAG9

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
24.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for EBAG9/RCAS1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for EBAG9/RCAS1 Overexpression Lysate (Native)

  • Cancer-associated surface antigen RCAS1
  • EB9cancer associated surface antigen
  • estrogen receptor binding site associated, antigen, 9
  • Estrogen receptor-binding fragment-associated gene 9 protein
  • RCAS1PDAF
  • receptor-binding cancer antigen expressed on SiSo cells

Background

This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor which binds to the estrogen-responsive element (ERE) found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript. Both variants (1 and 2) encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for EBAG9/RCAS1 Lysate (NBL1-10090) (0)

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Video Protocols

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Bioinformatics

Gene Symbol EBAG9