EB3 Antibody

Images

 
Western Blot: EB3 Antibody [NBP1-52003] - Staining of Human (A), Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: EB3 Antibody [NBP1-52003] - Staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: EB3 Antibody [NBP1-52003] - (0.05ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

EB3 Antibody Summary

Immunogen
Peptide with sequence C-KLIGTAVPQRTSPT corresponding to internal region according to NP_036458.2.
Epitope
C-KLIGTAVPQRTSPT
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MAPRE3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 33 kDa band observed in human (cerebellum), mouse and rat brain lysates (calculated MW of 32.0 kDa band according to human NP_036458.2, mouse NP_579928.1 and rat NP_001007657.1).
Publications
Read Publication using NBP1-52003.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EB3 Antibody

  • EB1 protein family member 3
  • EB3EBF3-S
  • End-binding protein 3
  • microtubule-associated protein RP/EB family member 3
  • microtubule-associated protein, RP/EB family, member 3
  • RP3EBF3APC binding protein

Background

The protein encoded by this gene is a member of the RP/EB family of genes. The protein localizes to the cytoplasmic microtubule network and binds APCL, a homolog of the adenomatous polyposis coli tumor suppressor gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EB3 Antibody (NBP1-52003)(1)


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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAPRE3
Entrez
Uniprot