EAAT3 Antibody (S180-41) [Alexa Fluor™ Plus 594]

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF, IP
• Clone: S180-41
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 594

EAAT3 Antibody (S180-41) [Alexa Fluor™ Plus 594] Summary

• Immunogen: Fusion protein amino acids 1-524 (full length) of human EAAT3 (Uniprot: P43005)
• Localization: Membrane
• Specificity: Detects 60kDa.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC1A1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for EAAT3 Antibody (S180-41) [Alexa Fluor™ Plus 594]

• EAAC1
• EAAC1excitatory amino acid carrier 1
• EAAT3
• EAAT3excitatory amino acid transporter 3
• Excitatory amino-acid carrier 1
• Neuronal and epithelial glutamate transporter
• SLC1A1
• Sodium-dependent glutamate/aspartate transporter 3
• solute carrier family 1 (neuronal/epithelial high affinity glutamatetransporter, system Xag), member 1
• Solute carrier family 1 member 1

Background

The EAAT3 gene encodes for glutamate transporters (also known as excitatory amino acid transporters, EAATs), that rapidly move glutamate from the postsynaptic cleft through the plasma membrane. The proteins are approximately 542 amino acids long at a mass barely over 57 kDA. This function allows for neurotoxic levels of glutamate in the brain to be avoided. It is common to see underactivity of glutamate receptors in some disorders such as ischemia and traumatic brain injuries and overactivity to be linked to mental illnesses such as schizophrenia. EAAT3 gene is also related to obsessive-compulsive disorder, ganglioglioma, autism spectrum disorders, aminoaciduriam temporal lobe epilepsy, hepatic encephalopathy, neuronitis, anorexia nervosa, and amyotrophic lateral sclerosis. It interacts with KNCN, HCCS, EWSR1, PRKCA, and ARL6IP5 to function in pathways of glutamic acid signaling, transmembrane transport of small molecules, and protein digestion and absorption.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC1A1