Home » EAAT3 » EAAT3 Antibodies » EAAT3 Antibody (JU39-69)

EAAT3 Antibody (JU39-69)

 Submit a Review

Images

 
(
Enlarge)
Western Blot: EAAT3 Antibody (JU39-69) [NBP2-75476] - Analysis of EAAT3 on mouse liver tissue lysate using anti-EAAT3 antibody at 1/500 dilution.
Western Blot: EAAT3 Antibody (JU39-69) [NBP2-75476] - Analysis of EAAT3 on mouse liver tissue lysate using anti-EAAT3 antibody at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence: EAAT3 Antibody (JU39-69) [NBP2-75476] - Staining EAAT3 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised ...read more
Immunohistochemistry-Paraffin: EAAT3 Antibody (JU39-69) [NBP2-75476] - Analysis of paraffin-embedded rat brain tissue using anti-EAAT3 antibody. Counter stained with hematoxylin.
Immunocytochemistry/ Immunofluorescence: EAAT3 Antibody (JU39-69) [NBP2-75476] - Staining EAAT3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunocytochemistry/ Immunofluorescence: EAAT3 Antibody (JU39-69) [NBP2-75476] - Staining EAAT3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: EAAT3 Antibody (JU39-69) [NBP2-75476] - Analysis of paraffin-embedded human tonsil tissue using anti-EAAT3 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: EAAT3 Antibody (JU39-69) [NBP2-75476] - Analysis of paraffin-embedded human colon cancer tissue using anti-EAAT3 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: EAAT3 Antibody (JU39-69) [NBP2-75476] - Analysis of paraffin-embedded human kidney tissue using anti-EAAT3 antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
JU39-69
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

100% Guarantee on every product

EAAT3 Antibody (JU39-69) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Recombinant protein
Localization
Cell membrane.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
SLC1A1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for EAAT3 Antibody (JU39-69)

  • EAAC1excitatory amino acid carrier 1
  • EAAT3excitatory amino acid transporter 3
  • Excitatory amino-acid carrier 1
  • Neuronal and epithelial glutamate transporter
  • Sodium-dependent glutamate/aspartate transporter 3
  • solute carrier family 1 (neuronal/epithelial high affinity glutamatetransporter, system Xag), member 1
  • Solute carrier family 1 member 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...


EAAT1/GLAST-1/SLC1A3 A
NB100-1869
Flow (Intracellular): EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Intracellular staining of HEK293 cells (1 x 10^6 cells/mL) with SLC1A3 antibody (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).
Immunohistochemistry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Staining of SLC1A3 in rat cerebellum sections
Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Analysis of SLC1A3 in 1. Human brain 2. Mouse brain and 3. Rat brain whole cell lysates.
(1 Review)
(15 Publications)
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Flow-IC
EAAT2/GLT1
Antibody - Azide Free
NBP1-20136
Western Blot: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:1000 incubated overnight at 4C.
Immunocytochemistry/Immunofluorescence: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Human NPCs differentiate into mature neurons. Immunofluorescence staining of differentiated neurons derived from human dorsal NPCs (1323-2 line, day 35 after differentiation) for mature cortical neuronal markers expressed in the nucleus (BRN2, TBR1, NeuN) and cytoplasm (MAP2), glial markers (SOX9, GFAP, GLT1), and dorsal forebrain marker (FOXG1). Nuclei stained with DAPI, shown as an overlay over brightfield images. The merge is an overlay of the neuronal and glial markers. Scale bar 100 um. DAPI 4,6'-diamino-2-phenylindole Image collected and cropped by CiteAb from the following publication (//stemcellres.biomedcentral.com/articles/10.1186/s13287-018-0812-6) licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Sections of mouse olfactory bulbs. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.
(8 Publications)
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P, In vivo, ISH
EAAT4 Antibody
NBP2-48740
Immunocytochemistry/Immunofluorescence: EAAT4 Antibody [NBP2-48740] - Staining of human cell line A-431 shows localization to intermediate filaments.
Immunohistochemistry-Paraffin: EAAT4 Antibody [NBP2-48740] - Staining in human cerebral cortex and kidney tissues using anti-SLC1A6 antibody. Corresponding SLC1A6 RNA-seq data are presented for the same tissues.
Immunocytochemistry/Immunofluorescence: EAAT4 Antibody [NBP2-48740] - Staining of human cell line A-431 shows localization to intermediate filaments.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Complement C6
Antibody
NB120-11899
Complement C6 Antibody
Species: Mu
Applications: WB, IHC, IHC-Fr
PSMA7 Antibody
(1A10-3G12)
H00005688-M01
Western Blot: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of PSMA7 expression in transfected 293T cell line by PSMA7 monoclonal antibody (M01), clone 1A10-3G12.Lane 1: PSMA7 transfected lysate (Predicted MW: 27.9 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of monoclonal antibody to PSMA7 on HeLa cell. Antibody concentration 1 ~ 10 ug/ml.
Immunohistochemistry-Paraffin: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human colon tissue. Antibody concentration 5 ug/ml.
(1 Review)
(2 Publications)
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, S-ELISA
JWA Antibody
NB100-1105
Western Blot: JWA Antibody [NB100-1105] - Staining of Human Brain lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: JWA Antibody [NB100-1105] - Staining of Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: JWA Antibody [NB100-1105] - Staining of Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
(2 Publications)
Species: Hu, Mu
Applications: WB, IHC, IHC-P, PEP-ELISA
PRRT2 Antibody
NBP2-82026
Western Blot: PRRT2 Antibody [NBP2-82026] - Analysis of PRRT2 in mouse brain tissue lysate with PRRT2 antibody at 1 ug/ml.
Immunocytochemistry/Immunofluorescence: PRRT2 Antibody [NBP2-82026] - Immunofluorescence of PRRT2 in rat brain tissue with PRRT2 antibody at 20 ug/ml.
Immunohistochemistry: PRRT2 Antibody [NBP2-82026] - Immunohistochemistry of PRRT2 in rat brain tissue with PRRT2 antibody at 2.5 ug/ml.
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
SLC1A7 Antibody
NBP2-81802
Western Blot: SLC1A7 Antibody [NBP2-81802] - Analysis of SLC1A7 in C2C12 cell lysate with SLC1A7 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry/Immunofluorescence: SLC1A7 Antibody [NBP2-81802] - Immunofluorescence of SLC1A7 in mouse kidney tissue with SLC1A7 antibody at 20 ug/ml.
Immunohistochemistry: SLC1A7 Antibody [NBP2-81802] - Immunohistochemistry of SLC1A7 in mouse kidney tissue with SLC1A7 antibody at 5 ug/ml.
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Carbonic Anhydrase
I/CA1 Antibody
NBP1-88191
Western Blot: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Comparative proteome analysis of 3xTg-AD and control samples at different stages of AD. Immunoblot analysis of most regulated hits. Soluble fractions of brain proteins were analyzed from four 2-month-old control and 3xTg-AD mice animals, respectively. Hebp1 and Glo1 levels were consistently elevated in the transgenic animals as compared to wild type controls. Ca1 levels were reduced in transgenic animals. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/47498), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Staining of human rectum shows strong cytoplasmic and nuclear positivity in glandular cells.
Western Blot: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Analysis in human cell line HELA.
(3 Publications)
Species: Hu, Rt
Applications: WB, IHC, IHC-P
Glutamine
Synthetase Antibody
NB110-41404
Western Blot: Glutamine Synthetase Antibody [NB110-41404] - Analysis of glutamine synthase. 40ug of lysates from mouse (Lanes M), rat (Lane R), pig (Lane P), bovine (Lane B), or human (Lane Hu) retina were probed. A 42 kDa band was identified in lysates from retinas of all species.
Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody [NB110-41404] - Immunofluorescence using NB110-41404. Submitted via verified customer review.
Immunohistochemistry: Glutamine Synthetase Antibody [NB110-41404] - Localization of glutamine synthase in the retina. Paraffin sections of mouse (A, B), rat (C, D, G-I), or human (E, F) retina fixed in 4% paraformaldehyde were reacted with anti-glutamine synthase (red fluorescence staining in B, D, G, I, and brown immuno-peroxidase reaction. Nuclei in IF experiments (A-D) were stained with DAPI (cyan), and with nuclear fast red in E and F. At low magnification, anti-glutamine synthase reacted with a single population of cells extending from the ganglion cell layer through the inner nuclear layer. No signal was detected in controls either pre-incubated with 100ug/ml of the immunizing peptide (A) or with pre-immune serum (C, E). This finding was confirmed by co-localization (indicated by yellow in I) of glutamine synthase (red in G) with antoher marker of glutamine synthase (green in H).
(1 Review)
(6 Publications)
Species: Hu, Mu, Rt, Bv
Applications: WB, ICC/IF, IHC
GFAP Antibody
NB300-141
Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Analysis of mixed neuron-glial cultures using GFAP antibody NB300-141 (red) and Vimentin antibody NB300-223 (green). The fibroblastic cells contain only Vimentin and so are green. The astrocytes contain either Vimentin and GFAP (appearing golden) or predominantly GFAP (appearing red). Blue is nuclear DNA stain.
Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Rat neurons stained with Neurofilament Heavy antibody NB300-217 (red) and GFAP antibody NB300-141 (green).
Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Xenografted mouse brain section: astocyte and human nuclei. ICC/IF image submitted by a verified customer review.
(12 Reviews)
(60 Publications)
Species: Hu, Mu, Rt, Po, Bv, Ch, Eq, Gp, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
VGLUT1 Antibody
(CL2754)
NBP2-46627
Immunohistochemistry-Paraffin: VGLUT1 Antibody (CL2754) [NBP2-46627] - VGLUT1 Antibody (2754) [NBP2-46627] - Staining in human cerebral cortex and tonsil tissues. Corresponding VGLUT1 RNA-seq data are presented for the same tissues.
Western Blot: VGLUT1 Antibody (2754) [NBP2-46627] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue
Immunohistochemistry: VGLUT1 Antibody (2754) [NBP2-46627] - Staining of rat brain shows strong positivity in glutamatergic processes in the molecular and granular layers of cerebellum.
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
mGluR2/3 Antibody
NB300-124
Western Blot: metabotropic Glutamate Receptor 2/3 Antibody [NB300-124] - Western blot of 10 ug of rat hippocampal lysate showing the specific immunolabeling of the ~110k monomer and the ~220k dimer of mGluR2 and mGluR3.
Western Blot: metabotropic Glutamate Receptor 2/3 Antibody [NB300-124] - Western blot of 10 ug of HEK 293 cells expression. As shown in the autoradiograph, the mGluR2/3 antibody recognizes both the ~110k monomer and the ~220k dimer of mGluR2 (Lane A) and mGluR3 (Lane B). Also shown is the specific immunolabeling of the monomer
Western Blot: metabotropic Glutamate Receptor 2/3 Antibody [NB300-124] - Specificity of mGluR2/3 (NB 300-124) in rat tissue lysate.
(4 Publications)
Species: Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr
PEPT1/SLC15A1
Antibody
NBP1-92005
Immunocytochemistry/Immunofluorescence: PEPT1/SLC15A1 Antibody [NBP1-92005] - Staining of human cell line CACO-2 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PEPT1/SLC15A1 Antibody [NBP1-92005] - Staining of human small intestine shows strong membranous and moderate cytoplasmic positivity in glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
GluR2 Antibody
(JU20-43)
NBP2-75510
Immunocytochemistry/Immunofluorescence: GluR2 Antibody (JU20-43) [NBP2-75510] - Staining GluR2 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: GluR2 Antibody (JU20-43) [NBP2-75510] - Analysis of paraffin-embedded mouse brain tissue using anti-GluR2 antibody. Counter stained with hematoxylin.
Immunocytochemistry/Immunofluorescence: GluR2 Antibody (JU20-43) [NBP2-75510] - Staining GluR2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
PIK3CA Antibody
NBP2-19804
Western Blot: PIK3CA Antibody [NBP2-19804] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 alpha antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: PIK3CA Antibody [NBP2-19804] - HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PI3 kinase p110 alpha protein stained by PI3 kinase p110 alpha antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: PIK3CA Antibody [NBP2-19804] - PIK3CA Antibody Immunohistochemical analysis of paraffin-embedded Human Breast Cancer, using PI3 kinase p110 alpha antibody.
(1 Publication)
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
PI 3-Kinase p110
beta/PIK3CB Antibody ...
NBP2-88937
Western Blot: PI 3-Kinase p110 beta/PIK3CB Antibody (CL9518) [NBP2-88937] - Analysis in human cell line HEL.
Immunocytochemistry/Immunofluorescence: PI 3-Kinase p110 beta/PIK3CB Antibody (CL9518) [NBP2-88937] - Staining of A-431 cells using the Anti-PIK3CB monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: PI 3-Kinase p110 beta/PIK3CB Antibody (CL9518) [NBP2-88937] - Staining of human thyroid gland shows strong nuclear positivity in glandular cells.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
EAAT3 Antibody
(JU39-69)
NBP2-75476
EAAT3 Antibody (JU39-69)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Publications for EAAT3 Antibody (NBP2-75476) (0)

There are no publications for EAAT3 Antibody (NBP2-75476).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for EAAT3 Antibody (NBP2-75476) (0)

There are no reviews for EAAT3 Antibody (NBP2-75476). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EAAT3 Antibody (NBP2-75476) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Learn about Custom Antibody Labeling

Additional EAAT3 Products

Bioinformatics Tool for EAAT3 Antibody (NBP2-75476)

Discover related pathways, diseases and genes to EAAT3 Antibody (NBP2-75476). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for EAAT3 Antibody (NBP2-75476)

Discover more about diseases related to EAAT3 Antibody (NBP2-75476).
 

Pathways for EAAT3 Antibody (NBP2-75476)

View related products by pathway.

PTMs for EAAT3 Antibody (NBP2-75476)

Learn more about PTMs related to EAAT3 Antibody (NBP2-75476).

Blogs on EAAT3

There are no specific blogs for EAAT3, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our EAAT3 Antibody (JU39-69) and receive a gift card or discount.


Bioinformatics

Gene Symbol SLC1A1