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EAAT3 Antibody (JU39-69)

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Western Blot: EAAT3 Antibody (JU39-69) [NBP2-75476] - Analysis of EAAT3 on mouse liver tissue lysate using anti-EAAT3 antibody at 1/500 dilution.
Western Blot: EAAT3 Antibody (JU39-69) [NBP2-75476] - Analysis of EAAT3 on mouse liver tissue lysate using anti-EAAT3 antibody at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence: EAAT3 Antibody (JU39-69) [NBP2-75476] - Staining EAAT3 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised ...read more
Immunohistochemistry-Paraffin: EAAT3 Antibody (JU39-69) [NBP2-75476] - Analysis of paraffin-embedded rat brain tissue using anti-EAAT3 antibody. Counter stained with hematoxylin.
Immunocytochemistry/ Immunofluorescence: EAAT3 Antibody (JU39-69) [NBP2-75476] - Staining EAAT3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunocytochemistry/ Immunofluorescence: EAAT3 Antibody (JU39-69) [NBP2-75476] - Staining EAAT3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: EAAT3 Antibody (JU39-69) [NBP2-75476] - Analysis of paraffin-embedded human tonsil tissue using anti-EAAT3 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: EAAT3 Antibody (JU39-69) [NBP2-75476] - Analysis of paraffin-embedded human colon cancer tissue using anti-EAAT3 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: EAAT3 Antibody (JU39-69) [NBP2-75476] - Analysis of paraffin-embedded human kidney tissue using anti-EAAT3 antibody. Counter stained with hematoxylin.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-75476
    • Availability
      Product Discontinued

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EAAT3 Antibody (JU39-69) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Recombinant protein within Human EAAT3 aa 80-209 / 524. (SwissProt: P43005 Human; SwissProt: P51906 Mouse; SwissProt: P51907 Rat)
Localization
Cell membrane.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
SLC1A1
Purity
Protein A purified
Innovator's Reward
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Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for EAAT3 Antibody (JU39-69)

  • EAAC1
  • EAAC1excitatory amino acid carrier 1
  • EAAT3
  • EAAT3excitatory amino acid transporter 3
  • Excitatory amino-acid carrier 1
  • Neuronal and epithelial glutamate transporter
  • SLC1A1
  • Sodium-dependent glutamate/aspartate transporter 3
  • solute carrier family 1 (neuronal/epithelial high affinity glutamatetransporter, system Xag), member 1
  • Solute carrier family 1 member 1

Background

The EAAT3 gene encodes for glutamate transporters (also known as excitatory amino acid transporters, EAATs), that rapidly move glutamate from the postsynaptic cleft through the plasma membrane. The proteins are approximately 542 amino acids long at a mass barely over 57 kDA. This function allows for neurotoxic levels of glutamate in the brain to be avoided. It is common to see underactivity of glutamate receptors in some disorders such as ischemia and traumatic brain injuries and overactivity to be linked to mental illnesses such as schizophrenia. EAAT3 gene is also related to obsessive-compulsive disorder, ganglioglioma, autism spectrum disorders, aminoaciduriam temporal lobe epilepsy, hepatic encephalopathy, neuronitis, anorexia nervosa, and amyotrophic lateral sclerosis. It interacts with KNCN, HCCS, EWSR1, PRKCA, and ARL6IP5 to function in pathways of glutamic acid signaling, transmembrane transport of small molecules, and protein digestion and absorption.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on Hek293 cells with EAAT1/GLAST-1/SLC1A3 antibody NB100-1869PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on Hek293 cells with EAAT1/GLAST-1/SLC1A3 Antibody NB100-1869AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on U937 cells with SLC1A3 Antibody NB100-1869 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).
(1 Review)
(22 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB
EAAT2/GLT1
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NBP1-20136
Western Blot: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:1000 incubated overnight at 4C.
Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.
Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.
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Species: Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, In vivo, ISH, WB
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Immunohistochemistry-Frozen: EAAT4 Antibody [NBP3-13360] - EAAT4 antibody detects EAAT4 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: EAAT4 stained by EAAT4 antibody (NBP3-13360) diluted at 1:250. Red: NF-H, stained by NF-H antibody [GT114] diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: EAAT4 Antibody [NBP3-13360] - EAAT4 antibody detects EAAT4 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. EAAT4 stained by EAAT4 antibody (NBP3-13360) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunocytochemistry/Immunofluorescence: EAAT4 Antibody [NBP3-13360] - EAAT4 antibody detects EAAT4 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EAAT4 stained by EAAT4 antibody (NBP3-13360) diluted at 1:500. Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:500. Blue: Fluoroshield with DAPI.
Species: Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P
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Western Blot: Complement C6 Antibody [NBP1-91803] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted)
Immunohistochemistry-Paraffin: Complement C6 Antibody [NBP1-91803] - Staining of human prostate shows strong granular cytoplasmic and membranous positivity in glandular cells.
Species: Hu
Applications: IHC, IHC-P, WB
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Western Blot: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of PSMA7 expression in transfected 293T cell line by PSMA7 monoclonal antibody (M01), clone 1A10-3G12.Lane 1: PSMA7 transfected lysate (Predicted MW: 27.9 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of monoclonal antibody to PSMA7 on HeLa cell. Antibody concentration 1 ~ 10 ug/ml.
Immunohistochemistry-Paraffin: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. Antibody concentration 5 ug/ml.
(1 Review)
(2 Publications)
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, S-ELISA, WB
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NBP3-12158
Western Blot: JWA Antibody [NBP3-12158] - Staining of Human Cerebellum lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Western Blot: JWA Antibody [NBP3-12158] - Staining of K562 cell lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/Immunofluorescence: JWA Antibody [NBP3-12158] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing plasma membrane and ER/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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Immunohistochemistry: PRRT2 Antibody [NBP2-82026] - Immunohistochemistry of PRRT2 in rat brain tissue with PRRT2 antibody at 2.5 ug/ml.
Immunocytochemistry/Immunofluorescence: PRRT2 Antibody [NBP2-82026] - Immunofluorescence of PRRT2 in rat brain tissue with PRRT2 antibody at 20 ug/ml.
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
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BSA Free
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Western Blot: SLC1A7 Antibody [NBP2-81802] - Analysis of SLC1A7 in C2C12 cell lysate with SLC1A7 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunocytochemistry/Immunofluorescence: SLC1A7 Antibody [NBP2-81802] - Immunofluorescence of SLC1A7 in mouse kidney tissue with SLC1A7 antibody at 20 ug/ml.
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
Carbonic Anhydrase
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Western Blot: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Analysis in human cell line HELA.
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(1 Review)
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Western Blot: GFAP Antibody [NB300-141] - Analysis of different tissue lysates using rabbit polyclonal antibody to GFAP, NB300-141, dilution 1:5000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Strong band at about 50 kDa corresponds to the major isotype of the GFAP protein. Smaller isotypes and proteolytic fragments of GFAP are also detected on the blot.
Western Blot: GFAP Antibody [NB300-141] - Analysis of Rat brain lysate. Antibody at 1:5000. Specific band running with an apparent SDS-PAGE molecular weight of ~50 kDa corresponds to rodent GFAP was observed.
(12 Reviews)
(83 Publications)
Species: Bv, Ch, Eq, Gp, Hu, Mu, Po, Rb, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
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Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP3-13165] - Rat brain. VGLUT1 antibody (NBP3-13165) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Frozen: VGLUT1 Antibody [NBP3-13165] - VGLUT1 antibody detects VGLUT1 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: VGLUT1 protein stained by VGLUT1 antibody (NBP3-13165) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.
Western Blot: VGLUT1 Antibody [NBP3-13165] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VGLUT1 antibody (NBP3-13165) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
mGluR2/3 Antibody
NB300-124
Western Blot: metabotropic Glutamate Receptor 2/3 Antibody [NB300-124] - Western blot of 10 ug of HEK 293 cells expression. As shown in the autoradiograph, the mGluR2/3 antibody recognizes both the ~110k monomer and the ~220k dimer of mGluR2 (Lane A) and mGluR3 (Lane B). Also shown is the specific immunolabeling of the monomer
Western Blot: metabotropic Glutamate Receptor 2/3 Antibody [NB300-124] - Specificity of mGluR2/3 (NB 300-124) in rat tissue lysate.
Western Blot: metabotropic Glutamate Receptor 2/3 Antibody [NB300-124] - Western blot of 10 ug of rat hippocampal lysate showing the specific immunolabeling of the ~110k monomer and the ~220k dimer of mGluR2 and mGluR3.
(5 Publications)
Species: Mu, Rt
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PEPT1/SLC15A1
Antibody
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Immunocytochemistry/Immunofluorescence: PEPT1/SLC15A1 Antibody [NBP2-98544] - Immunofluorescence staining of PEPT1/SLC15A1 in CACO-2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PEPT1/SLC15A1 polyclonal antibody (dilution ratio 1:300) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.
Immunohistochemistry-Paraffin: PEPT1/SLC15A1 Antibody [NBP2-98544] - Staining of human PEPT1/SLC15A1 in human gallbladder with rabbit polyclonal antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: PEPT1/SLC15A1 Antibody [NBP2-98544] - Staining of human SLC15A1 in human small intestine with rabbit polyclonal antibody at 1:500 dilution.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
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(JU20-43)
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Immunocytochemistry/Immunofluorescence: GluR2 Antibody (JU20-43) [NBP2-75510] - Staining GluR2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: GluR2 Antibody (JU20-43) [NBP2-75510] - Staining GluR2 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: GluR2 Antibody (JU20-43) [NBP2-75510] - Analysis of paraffin-embedded mouse brain tissue using anti-GluR2 antibody. Counter stained with hematoxylin.
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
PIK3CA Antibody
NBP2-19804
Western Blot: PIK3CA Antibody [NBP2-19804] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 alpha antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunohistochemistry-Paraffin: PIK3CA Antibody [NBP2-19804] - Human Breast Cancer, using PI3 kinase p110 alpha antibody. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: PIK3CA Antibody [NBP2-19804] - PIK3CA Antibody Sample (50 ug of whole cell lysate) A: C2C12 B: C2C12+AEA10mM NBP2-19804 diluted at 1:1000.
(3 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
PI 3-Kinase p110
beta/PIK3CB Antibody ...
NBP2-88937
Immunocytochemistry/Immunofluorescence: PI 3-Kinase p110 beta/PIK3CB Antibody (CL9518) [NBP2-88937] - Staining of A-431 cells using the Anti-PIK3CB monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: PI 3-Kinase p110 beta/PIK3CB Antibody (CL9518) [NBP2-88937] - Staining of human cervix shows strong nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: PI 3-Kinase p110 beta/PIK3CB Antibody (CL9518) [NBP2-88937] - Staining of human colonshows strong nuclear positivity in glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
EAAT3 Antibody
(JU39-69)
NBP2-75476
EAAT3 Antibody (JU39-69)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

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Bioinformatics

Gene Symbol SLC1A1