HAF109). A specific band was detected for E2F-4 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human E2F-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5139) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for E2F-4 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5139-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26608-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98787-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26925 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58401-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26928 | |||
![Western Blot: E2F-4 Antibody [NBP1-21374] - Detection of Human E2F4 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15, 50 ug) and 293T (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-E2F4 antibody NBP1-21374 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/E2F-4-Antibody-Western-Blot-NBP1-21374-img0039.jpg "<b>Biological Strategies Validation. </b>Western Blot: E2F-4 Antibody [NBP1-21374] - Detection of Human E2F4 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15, 50 ug) and 293T (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-E2F4 antibody NBP1-21374 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: E2F-4 Antibody [NBP1-21374] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-E2F4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/E2F-4-Antibody-Immunohistochemistry-NBP1-21374-img0037.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: E2F-4 Antibody [NBP1-21374] - Detection of human E2F4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-E2F4 antibody NBP1-21374 used for IP at 6 ug per reaction. E2F4 was also immunoprecipitated by another rabbit anti-E2F4 antibody. For blotting immunoprecipitated E2F4, NBP1-21374 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/E2F-4-Antibody-Immunoprecipitation-NBP1-21374-img0040.jpg)
![Western Blot: E2F-4 Antibody (JE40-78) [NBP3-32285] - Western blot analysis of E2F-4 on different lysates with Rabbit anti-E2F-4 antibody (NBP3-32285) at 1/1,000 dilution.<br/><br/> Lane 1: K-562 cell lysate<br/> Lane 2: A549 cell lysate<br/> Lane 3: HEK-293 cell lysate<br/> Lane 4: Jurkat cell lysate<br/> Lane 5: HepG2 cell lysate<br/> Lane 6: Raji cell lysate<br/> Lane 7: HeLa cell lysate<br/><br/> Lysates/proteins at 15 ug/Lane.<br/><br/> Predicted band size: 44 kDa<br/> Observed band size: 60 kDa<br/><br/> Exposure time: 3 minutes;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32285) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32285_rabbit-e2f-4-mab-je40-78-278202416331292.jpg "Western Blot: E2F-4 Antibody (JE40-78) [NBP3-32285] - Western blot analysis of E2F-4 on different lysates with Rabbit anti-E2F-4 antibody (NBP3-32285) at 1/1,000 dilution.<br/><br/> Lane 1: K-562 cell lysate<br/> Lane 2: A549 cell lysate<br/> Lane 3: HEK-293 cell lysate<br/> Lane 4: Jurkat cell lysate<br/> Lane 5: HepG2 cell lysate<br/> Lane 6: Raji cell lysate<br/> Lane 7: HeLa cell lysate<br/><br/> Lysates/proteins at 15 ug/Lane.<br/><br/> Predicted band size: 44 kDa<br/> Observed band size: 60 kDa<br/><br/> Exposure time: 3 minutes;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32285) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: E2F-4 Antibody (JE40-78) [NBP3-32285] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-E2F-4 antibody (NBP3-32285) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32285) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32285_rabbit-e2f-4-mab-je40-78-298202414304159.jpg)
![Flow Cytometry: E2F-4 Antibody (JE40-78) [NBP3-32285] - Flow cytometric analysis of K-562 cells labeling E2F-4.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32285, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32285_rabbit-e2f-4-mab-je40-78-29202416372181.jpg)
![Immunohistochemistry: E2F-4 Antibody (24E12) [NBP3-26608] - Image of E2F-4 Antibody (24E12) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26608_rabbit-e2f-4-mab-24e12-262202414471533.jpg "Immunohistochemistry: E2F-4 Antibody (24E12) [NBP3-26608] - Image of E2F-4 Antibody (24E12) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Flow Cytometry: E2F-4 Antibody (24E12) [NBP3-26608] - Overlay Peak curve showing MCF7 cells stained with E2F-4 Antibody (24E12) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26608_rabbit-e2f-4-mab-24e12-282202414312787.jpg)
![Immunocytochemistry/Immunofluorescence: E2F-4 Antibody (24E12) [NBP3-26608] -Immunofluorescence staining of Hela cell with E2F-4 Antibody at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 513-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).](http://images.novusbio.com/fullsize/nbp3-26608_rabbit-e2f-4-mab-24e12-29120251248506.jpg)
![Western Blot: E2F-4 Antibody [NBP2-98787] - Lane A: K562 Whole Cell LysateLane B: Raji Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:44 kDaObserved band size:53 kDa](http://images.novusbio.com/fullsize/E2F-4-Antibody-Western-Blot-NBP2-98787-img0002.jpg "Western Blot: E2F-4 Antibody [NBP2-98787] - Lane A: K562 Whole Cell LysateLane B: Raji Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:44 kDaObserved band size:53 kDa")
![Immunocytochemistry/Immunofluorescence: E2F-4 Antibody [NBP2-98787] - Immunofluorescence staining of E2F-4 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98787 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-98787_rabbit-polyclonal-e2f-4-antibody-235202511444414.png)
![Western Blot: E2F-4 Antibody [H00001874-D01P] - Analysis of E2F4 expression in mouse spleen.](http://images.novusbio.com/fullsize/E2F-4-Antibody-Western-Blot-H00001874-D01P-img0003.jpg "Western Blot: E2F-4 Antibody [H00001874-D01P] - Analysis of E2F4 expression in mouse spleen.")
![Western Blot: E2F-4 Antibody [H00001874-D01P] - Analysis of E2F4 expression in transfected 293T cell line by E2F4 polyclonal antibody.Lane 1: E2F4 transfected lysate(44.00 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/E2F-4-Antibody-Western-Blot-H00001874-D01P-img0004.jpg)
![Immunohistochemistry: E2F-4 Antibody [NBP3-10895] - Immunohistochemical analysis of human intestine.](http://images.novusbio.com/fullsize/E2F-4-Antibody-Immunohistochemistry-NBP3-10895-img0001.jpg "Immunohistochemistry: E2F-4 Antibody [NBP3-10895] - Immunohistochemical analysis of human intestine.")
![Western Blot: E2F-4 Antibody (5B7) [H00001874-M01] - E2F4 monoclonal antibody (M01), clone 5B7 Analysis of E2F4 expression in A-549.](http://images.novusbio.com/fullsize/E2F-4-Antibody-5B7-Western-Blot-H00001874-M01-img0004.jpg "Western Blot: E2F-4 Antibody (5B7) [H00001874-M01] - E2F4 monoclonal antibody (M01), clone 5B7 Analysis of E2F4 expression in A-549.")
![Sandwich ELISA: E2F-4 Antibody (5B7) [H00001874-M01] - Detection limit for recombinant GST tagged E2F4 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/E2F-4-Antibody-5B7-Sandwich-ELISA-H00001874-M01-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: E2F-4 Antibody [NBP2-58401] - Staining of human cell line HeLa shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/E2F-4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58401-img0001.jpg "Immunocytochemistry/Immunofluorescence: E2F-4 Antibody [NBP2-58401] - Staining of human cell line HeLa shows localization to nucleoplasm.")
![Western Blot: E2F-4 Antibody (3O6Y9) [NBP3-15850] - Western blot analysis of extracts of various cell lines, using E2F-4 antibody (NBP3-15850) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/E2F-4-Antibody-3O6Y9-Western-Blot-NBP3-15850-img0001.jpg "Western Blot: E2F-4 Antibody (3O6Y9) [NBP3-15850] - Western blot analysis of extracts of various cell lines, using E2F-4 antibody (NBP3-15850) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
