E2F-4 Antibody (S09-7E6)

Western Blot: E2F-4 Antibody (S09-7E6) [NBP3-19623] - Western blot detection of E2F-4 in K562 cell lysates using NBP3-19623 (1:1000 diluted). Predicted band size: 44kDa. Observed band size: 65kDa.

• Reactivity: Hu
• Applications: WB
• Clone: S09-7E6
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

E2F-4 Antibody (S09-7E6) Summary

• Description: Novus Biologicals Rabbit E2F-4 Antibody (S09-7E6) (NBP3-19623) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human E2F-4 (Uniprot # Q16254)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: E2F4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/1000
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for E2F-4 Antibody (S09-7E6)

• E2F transcription factor 4, p107/p130-binding
• E2F4
• E2F-4
• E2F-4p107/p130-binding protein
• transcription factor E2F4

Background

E2F4 is a member of the E2F family of transcription factors. It binds DNA at the E2 recognition site found in the promoter region of a number of genes involved in cell cycle regulation and DNA replication. E2F4 binds to pRb, p107, and p130. Via its interaction with p130, E2F4 plays an important role in maintaining G2 arrest during G2 phase checkpoint activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: E2F4