E2F-1 Antibody (10D1 2B5)

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Immunocytochemistry/ Immunofluorescence: E2F1 Antibody (2B5) [NB600-208] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using E2F1 antibody at 1:100 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IP
Clone
10D1 2B5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

E2F-1 Antibody (10D1 2B5) Summary

Immunogen
The region encoding the DP-1 binding site (amino acids 223-332) of E2F-1 expressed in E. coli.
Specificity
This is specific for human E2F-1.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
E2F1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunoprecipitation
Application Notes
WB: Predicted MW is 47 kDa.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for E2F-1 Antibody (10D1 2B5)

  • E2F transcription factor 1
  • E2F1
  • E2F-1
  • PBR3
  • PRB-binding protein E2F-1
  • RBAP1
  • RBAP-1
  • RBBP3
  • RBBP-3
  • RBP3
  • Retinoblastoma-associated protein 1
  • Retinoblastoma-binding protein 3
  • transcription factor E2F1

Background

Clone 2B5 recognizes E2F-1 that was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter. The original member of the E2F family of transcription factors, now consisting of five, was identified through its association with the retinoblastoma protein, which controls its activity. E2F-1 is cell cycle regulated with very low levels in early G1 then increasing levels as cells move from G1 to S with highest levels of protein at the G1/S boundary, which is consistent with its role in S-phase entry.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol E2F1
Entrez