Dystrobrevin alpha Antibody (OTI1B2)

Western Blot: Dystrobrevin alpha Antibody (1B2) [NBP2-03422] Analysis of extracts (35ug) from 9 different cell lines by using anti-Dystrobrevin alpha monoclonal antibody.

Immunocytochemistry/Immunofluorescence: Dystrobrevin alpha Antibody (1B2) [NBP2-03422] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Dystrobrevin alpha.

Flow Cytometry: Dystrobrevin alpha Antibody (1B2) [NBP2-03422] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Dystrobrevin alpha antibody, and then analyzed by flow cytometry.

Western Blot: Dystrobrevin alpha Antibody (1B2) [NBP2-03422] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Dystrobrevin alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Dystrobrevin alpha.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF
• Clone: OTI1B2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.72 mg/ml

Dystrobrevin alpha Antibody (OTI1B2) Summary

• Description: Novus Biologicals Mouse Dystrobrevin alpha Antibody (OTI1B2) (NBP2-03422) is a monoclonal antibody validated for use in WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human DTNA (NP_116761) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: DTNA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:500-2000
• Theoretical MW: 58.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.72 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Dystrobrevin alpha Antibody (OTI1B2)

• Alpha-dystrobrevin
• D18S892EDTN
• DRP3FLJ96209
• DTN-1
• DTN-2
• DTN-3
• DTN-A
• dystrobrevin alpha
• dystrobrevin, alpha
• Dystrophin-related protein 3
• LVNC1

Background

The protein encoded by this gene belongs to the dystrobrevin subfamily of the dystrophin family. This protein is a component of the dystrophin-associated protein complex (DPC), which consists of dystrophin and several integral and peripheral membrane proteins, including dystroglycans, sarcoglycans, syntrophins and alpha- and beta-dystrobrevin. The DPC localizes to the sarcolemma and its disruption is associated with various forms of muscular dystrophy. Mutations in this gene are associated with left ventricular noncompaction with congenital heart defects. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DTNA
• Entrez:
  • 1837(Human)