Dysferlin Antibody (CL10888) - Azide and BSA Free

Analysis in human spleen and skin tissues using NBP3-43895 antibody. Corresponding Dysferlin RNA-seq data are presented for the same tissues.

Staining of human lymph node shows strong membranous positivity in non-germinal center cells and endothelial cells.

Staining of human skin shows no positivity in keratinocytes as expected.

Staining of human placenta shows moderate membranous positivity in trophoblastic cells.

Staining of human spleen shows moderate positivity in cells in red pulp.

Analysis in human cell line RH-30.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL10888
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Dysferlin Antibody (CL10888) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Dysferlin Antibody (CL10888) - Azide and BSA Free (NBP3-07994) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of O75923, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DYSF
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Dysferlin Antibody (CL10888) - Azide and BSA Free

• dysferlin
• dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive)
• dystrophy-associated fer-1-like 1
• FER1L1Dystrophy-associated fer-1-like protein
• Fer-1-like protein 1
• FLJ00175
• FLJ90168
• LGMD2B

Background

Dysferlin is the protein product of the 2p13 gene that is defective in patients with Limb-Girdle Muscular Dystrophy type 2B (LGMD2B) and Miyoshi Myopathy (MM). Dysferlin is normally localized to the muscle plasma membrane. In patients with LGMD2B and MM, immunoreactivity to dysferlin is severely reduced or lost, depending on the type of mutation. This antibody is used for the characterization of LGMD2B and MM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DYSF