DYRK3 Antibody

Images

 
Western Blot: DYRK3 Antibody [NB110-94876] - Detection of DYRK3 in Jurkat nuclear extracts using NB110-94876.
Immunocytochemistry/ Immunofluorescence: DYRK3 Antibody [NB110-94876] - DYRK3 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more

Product Details

Summary
Reactivity Hu, Pm, Mu(-), Rt(-), BvSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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Catalog# & Conjugate Size Price

DYRK3 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of human DYRK3 (within residues 500-600). [Swiss-Prot# O43781]
Localization
Nucleus.
Predicted Species
Bovine (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DYRK3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:50
Application Notes
This DYRK3 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen ~65 kDa. In ICC/IF distinct nuclear staining along with general cytoplasmic staining was observed in Hela cells.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Jurkat Lysate (NB820-89388)

Reactivity Notes

Human and monkey. Does not react with mouse and rat. Immunogen sequence has 94% homology to bovine.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DYRK3 Antibody

  • dual specificity tyrosine-phosphorylation-regulated kinase 3
  • dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3
  • dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 5
  • DYRK3
  • DYRK5
  • EC 2.7.12
  • EC 2.7.12.1
  • hYAK3-2
  • protein kinase Dyrk3
  • REDK
  • REDKRED
  • Regulatory erythroid kinase
  • YAK3-2

Background

DYRK3, or dual specificity tyrosine-phosphorylation-regulated kinase 3, is a negative regulator of EPO-dependent erythropoiesis and may place an upper limit on red cell production during stress erythropoiesis. DYRK3 inhibits cell death due to cytokine withdrawal in hematopoietic progenitor cells, and may also act by regulating CREB/CRE signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for DYRK3 Antibody (NB110-94876): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DYRK3 Antibody (NB110-94876). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in Cat.No. NB110-94876 (anti-DYRK3) but would like to know if you have tested or if any feedback is available on the testing of this antibody with siRNA for DYRK3?
    • With regard to NB110-94876, we have not yet tested this product with an siRNA knockout or knockdown sample. Thus far, this antibody has been evaluated in western blot with Jurkat whole cell lysate and ICC with HeLa cells. We have also not had any publications submitted to us using this product.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 405 Labeled NB110-94876V
FITC Labeled NB110-94876F

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Bioinformatics

Gene Symbol DYRK3
Entrez
Uniprot