Dynamin 3 Antibody

Western Blot: Dynamin 3 Antibody [NB120-3458] - Analysis of rat liver extract.

Immunocytochemistry/Immunofluorescence: Dynamin 3 Antibody [NB120-3458] - Immunofluorescence analysis of Dynamin III was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton(TM) X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Dynamin III Rabbit Polyclonal Antibody at 2 ug/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal(TM) Secondary Antibody, Alexa Fluor(R) 488 conjugate for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade(R) Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Dynamin 3 Antibody Summary

• Immunogen: Synthetic Peptide: C P(623) D K S F T E N D E N G Q A E N F(639)
• Specificity: Detects a band at approximately 100 kDa.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DNM3
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:250
  • Western Blot 1:1000
• Control:

  Dynamin 3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles. After opening store under sterile conditions.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Purity: Ammonium sulfate precipitation

Alternate Names for Dynamin 3 Antibody

• Dyna III
• dynamin 3
• dynamin family member
• dynamin-3
• EC 3.6.5.5
• MGC70433
• T-dynamin
• testicular

Background

The dynamins are a family of 100 kDa GTPases transcribed from at least three separate genes. At least four mRNA splice variants for each dynamin have been described. Dynamins contain several conserved regions including the conserved, amino-terminal GTPase domain, a centrally located membrane-binding plekstrin homology domain (PHD), and a coiled-coil region located in front of a proline-rich domain (PRD). The PRD is thought to mediate interactions between dynamin and numerous other cellular proteins. Dynamin 1 is expressed exclusively in neurons, Dynamin 2 is ubiquitously expressed, and Dynamin 3 is thought to be restricted to expression in the brain, testis, heart, and lung. The dynamins participate in the cellular process of clathrin-mediated and fluid-phase endocytosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dynamin 3 Antibody (NB120-3458)(2)

Publications using NB120-3458

• Kolokoltsov, rey A et al. Small Interfering RNA Profiling Reveals Key Role of Clathrin-Mediated Endocytosis Early Endosome Formation for Infection by Respiraory Syncytial Virus. 81: 7786-7800, 2007. [PMID: 17494077]
• Kelly BL, Vassar R, Ferreira A et al. Beta-amyloid-induced dynamin 1 depletion in hippocampal neurons. A potential mechanism for early cognitive decline in Alzheimer disease. J Biol Chem. 2005-09-01 [PMID: 16002400]

Bioinformatics

• Gene Symbol: DNM3
• Entrez:
  • 26052(Human)
  • 171574(Rat)