DUSP7 Antibody

Western Blot: DUSP7 Antibody [NBP1-31232] - Sample (30ug whole cell lysate) A:Raji 10% SDS PAGE, antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: DUSP7 Antibody [NBP1-31232] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: DUSP7 Antibody [NBP1-31232] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:100 dilution.

• Reactivity: Hu, Mu, Bv, Xp, Ze
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

DUSP7 Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human DUSP7. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Mouse (100%), Zebrafish (100%), Bovine (100%), Xenopus (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DUSP7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus Tropicalis (100%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for DUSP7 Antibody

• DKFZp586F2224
• dual specificity phosphatase 7
• Dual specificity protein phosphatase PYST2
• dual-specificity phosphatase-7
• EC 3.1.3.16
• EC 3.1.3.48
• MKPX
• MKP-X
• PYST2dual specificity protein phosphatase 7

Background

Dual-specificity phosphatases (DUSPs) constitute a large heterogeneous subgroup of the type I cysteine-basedprotein-tyrosine phosphatase superfamily. DUSPs are characterized by their ability to dephosphorylate both tyrosineand serine/threonine residues. DUSP7 belongs to a class of DUSPs, designated MKPs, that dephosphorylate MAPK(mitogen-activated protein kinase) proteins ERK (see MIM 601795), JNK (see MIM 601158), and p38 (see MIM 600289) withspecificity distinct from that of individual MKP proteins. MKPs contain a highly conserved C-terminal catalytic domainand an N-terminal Cdc25 (see MIM 116947)-like (CH2) domain. MAPK activation cascades mediate various physiologicprocesses, including cellular proliferation, apoptosis, differentiation, and stress responses (summary by Patterson etal., 2009 (PubMed 19228121)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: DUSP7