DUSP16 Antibody

Western Blot: DUSP16 Antibody [NBP1-33747] - Sample (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: DUSP16 Antibody [NBP1-33747] - Paraformaldehyde-fixed A431, using DUSP16 (NBP1-33747) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.

Immunohistochemistry-Paraffin: DUSP16 Antibody [NBP1-33747] - Paraffin-embedded mouse prostate. DUSP16 antibody [C2C3], C-term dilution: 1:500.

Immunohistochemistry-Paraffin: DUSP16 Antibody [NBP1-33747] - Paraffin-embedded mouse middle brain. DUSP16 antibody [C2C3], C-term dilution: 1:500.

Product Discontinued

DUSP16 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human DUSP16. The exact sequence is proprietary.
• Localization: Cytoplasm, Nucleus
• Predicted Species: Rat (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DUSP16
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 73 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for DUSP16 Antibody

• dual specificity phosphatase 16
• EC 3.1.3.16
• EC 3.1.3.48
• FLJ36298
• FLJ40991
• KIAA1700MGC129701
• MAP kinase phosphatase 7
• MAPK phosphatase-7
• Mitogen-activated protein kinase phosphatase 7
• MKP7dual specificity protein phosphatase 16
• MKP-7MGC129702

Background

The activation of mitogen-activated protein kinase (MAPK) cascades transduces various extracellular signals to the nucleus to induce gene expression, cell proliferation, differentiation, cell cycle arrest, and apoptosis. For full activation of MAPKs, dual-specificity kinases phosphorylate both threonine and tyrosine residues in MAPK TXY motifs. MKPs are dual-specificity phosphatases that dephosphorylate the TXY motif, thereby negatively regulating MAPK activity.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for DUSP16 Antibody (NBP1-33747)(1)

Publication using NBP1-33747

• Bose AK, Janes KA. A high-throughput assay for phosphoprotein-specific phosphatase activity in cellular extracts Mol Cell Proteomics 2012-12-11 [PMID: 23233447] (WB, Human)

Bioinformatics

• Gene Symbol: DUSP16
• Entrez:
  • 80824(Human)
• Uniprot:
  • Q9BY84(Human)