DUSP13 Antibody

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Western Blot: DUSP13 Antibody [NB100-56491] - Analysis of DUSP13 in human skeletal muscle lysate in the 1) absence and 2) presence of immunizing peptide using this antibody at 5 ug/ml.

Product Details

Summary
Reactivity Hu, Rt, Bv, Ca, Pm, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

DUSP13 Antibody Summary

Immunogen
A portion of amino acids 150-200 of human DUSP13 was used as the immunogen.
Specificity
The amino acid sequence used as immunogen is 100% homologous in human, rhesus monkey, chimp, dog and bovine, and 93% homologous in mouse and rat. It is 100% conserved in human isoforms 1, 2 and 3.
Predicted Species
Mouse (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DUSP13
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
Reviewed Applications
Read 1 Review rated 3
using
NB100-56491 in the following application:

Reactivity Notes

Reacts with Bovine, Chimpanzee, Dog, Human and Rhesus Monkey. 93% sequence identity with Mouse. Rat data from customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.2% gelatin
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for DUSP13 Antibody

  • Branching-enzyme interacting DSP
  • dual specificity phosphatase 13
  • Dual specificity phosphatase SKRP4
  • dual specificity protein phosphatase 13 isoform MDSP
  • dual specificity protein phosphatase 13
  • dual-specificity phosphatase SKRP4
  • DUSP13A
  • DUSP13B
  • EC 3.1.3.16
  • EC 3.1.3.48
  • FLJ32450
  • MDSPbranching-enzyme interacting dual-specificity protein phosphatase
  • Muscle-restricted DSP
  • Testis- and skeletal-muscle-specific DSP
  • TMDPBEDPSKRP4

Background

DUSP13, a novel DSP, is a member of highly conserved Protein-tyrosine phosphatase super family. Members of this family cooperate with protein kinases and play a vital role in cell cycle regulation. DUSP13 consists of a conserved catalytic domain and is expressed specifically in the testis and skeletal muscle. Based on mRNA localization and expression studies during development, DUSP13 is suggested to be expressed as 3 isoforms due to alternative splicing. Reports suggest DUSP13 might be involved in the regulation of meiosis and differentiation of testicular germ cells during spermatogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB
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Review for DUSP13 Antibody (NB100-56491) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB100-56491:
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Images Ratings Applications Species Date Details
  3
reviewed by:
Matt Stratton
WB Rat 06/11/2014
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Summary

ApplicationWestern Blot
Sample TestedNeonatal rat ventricular myocytes
SpeciesRat
LotNB100-56491SS
CommentsBand at apropriate moleculat weight although higher molecular weight bands are stronger

Blocking

Blocking Details5% milk in TBST, 1 hour at room temp

Primary Anitbody

Dilution Ratio1:1000, overnight, 4C, 2.5% BSA in TBST

Secondary Antibody

Secondary Descriptiongoat anti rabbit IgG HRP
Secondary Manufacturer Cat#Southern Biotech
Secondary Concentration1:2000

Details

Detection NotesChemiluninescent, west pico

Comments

CommentsBand at apropriate moleculat weight although higher molecular weight bands are stronger

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DUSP13 Antibody (NB100-56491) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on DUSP13

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Recent Reviews

3
5
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4
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3
1
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1
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Matt Stratton
06/11/2014
Application: WB
Species: Rat

Bioinformatics

Gene Symbol DUSP13
Entrez
Uniprot