DRP2 Overexpression Lysate

Western Blot: DRP2 Overexpression Lysate (Adult Normal) [NBP2-08164] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DRP2.

• Reactivity: Hu
• Applications: WB

DRP2 Overexpression Lysate Summary

• Description:

  DRP2 Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC215641
  
  Accession#: NM_001939
  
  Protein Tag: C-MYC/DDK
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
• Gene: DRP2

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct. Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
• Theoretical MW: 107.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for DRP2 Overexpression Lysate

• dystrophin related protein 2
• dystrophin-related protein 2
• MGC133255

Background

Dystrophin, utrophin and dystrophin-related protein 2 (DRP2) are Actin-binding proteins that are involved in anchoring the cytoskeleton to the plasma membrane. Dystrophin is the protein product of the Duchenne/Becker muscular dystrophy gene. Dystrophin is expressed in muscle and brain tissues, where it is localized to the inner surface of the plasma membrane. Evidence suggests that the upregulation of utrophin (also known as DRP1) can reduce the dystrophic pathology. DRP2 is principally expressed in the brain and spinal cord. Analysis of DRP2 expression in rat brain on SDS-PAGE reveals a characteristic quartet of bands from 100-120 kDa. DRP2 exhibits a punctate staining pattern of rat neuronal dendrites and in neuropil. DRP2 forms a complex with dystroglycan at the surface of myelin-forming Schwann cells and may play a role in the terminal stages of myelinogenesis in the peripheral nervous system. The gene encoding human DRP2 maps to chromosome Xq22.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: DRP2