Drosha Antibody

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Western Blot: Drosha Antibody [NB100-55410] - (0.3 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Rt, Mu, Ca, PmSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Drosha Antibody Summary

Immunogen
Peptide with sequence C-KQTDKQKLAQRE corresponding to internal region according to NP_081075.2.
Epitope
KQTDKQKLAQRE
Specificity
This antibody also cross-reacts with human Ribonuclease III (GeneID 29102; NP_037367.2).
Predicted Species
Mouse (100%), Primate (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DROSHA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 150-160 kDa band observed in human liver lysates (calculated MW of 151 kDa band according to NP_081075.2).
Positive Control
Liver Lysate (NB820-59232)
Publications
Read Publication using NB100-55410.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 21931811).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Drosha Antibody

  • drosha, double-stranded RNA-specific endoribonuclease
  • drosha, ribonuclease type III
  • EC 3.1.26.3
  • ETOHI2
  • nuclear RNase III Drosha
  • p241
  • Protein Drosha
  • putative protein p241 which interacts with transcription factor Sp1
  • putative ribonuclease III
  • RANSE3L
  • ribonuclease 3
  • Ribonuclease III
  • ribonuclease III, nuclear
  • RN3nuclear
  • RNase III

Background

Members of the ribonuclease III superfamily of double-stranded (ds) RNA-specific endoribonucleases participate in diverse RNA maturation and decay pathways in eukaryotic and prokaryotic cells (Fortin et al., 2002). The RNase III Drosha is the core nuclease that executes the initiation step of microRNA (miRNA) processing in the nucleus (Lee et al., 2003).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Drosha Antibody (NB100-55410)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DROSHA
Entrez
Uniprot