Drebrin 1 Antibody (M2F6)

Western Blot: Drebrin 1 Antibody (M2F6) [NB100-1951] - Analysis of Drebrin expression in mouse brain (1) NIH/3T3 (2), Jurkat (3), HeLa (4), and PC12 (5)

Western Blot: Drebrin 1 Antibody (M2F6) [NB100-1951] - Analysis of mouse brain tissue extract probed with NB100-1951

Product Discontinued

Drebrin 1 Antibody (M2F6) Summary

• Immunogen: Chicken Drebrin E protein.
• Localization: Cytoplasmic
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DBN1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Immunoprecipitation
  • Western Blot 1ug/ml
• Application Notes: This antibody has been reported in the literature to work in IHC (PMID: 1314351).
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Mouse Brain Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 50% Glycerol
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Drebrin 1 Antibody (M2F6)

• D0S117E
• DBN1
• Developmentally-regulated brain protein
• DKFZp434D064
• Drba
• Drebrin 1
• Drebrin A
• Drebrin E
• Drebrin E2
• drebrin
• drebrin-1

Background

Drebrins are actin-binding proteins, that are developmentally regulated in the process of neuronal growth. Three isoforms, E1 and E2 (embryonic) and A (adult) are generated by alternative RNA splicing from a single debrin gene in the chicken brain. The expression pattern of each isoform is regulated spatially and temporally in the developing brain. It has been suggested that debrin is one of the intracellular regulators of the neuronal morphogenesis (4). Debrin has also been detected in other kinds of non-neuronal human and bovine tissue but its exact role is unknown.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Drebrin 1 Antibody (NB100-1951)(4)

Publications using NB100-1951

• Richter Katharina N, Wildhagen Hanna, Helm Martin S et al. Comparative synaptosome imaging: a semi-quantitative method to obtain copy numbers for synaptic and neuronal proteins. Scientific Reports 2018-10-04 [PMID: 30287847] (Rat)
• Bhat NR, Thirumangalakudi L. Increased Tau Phosphorylation and Impaired Brain Insulin/IGF Signaling in Mice Fed a High Fat/High Cholesterol Diet. J Alzheimers Dis 2013-05-23 [PMID: 23703152]
• Chen Q, Prior M, Dargusch R et al. A novel neurotrophic drug for cognitive enhancement and Alzheimer’s disease. PLoS ONE6 (12). 2011-01-01 [PMID: 22194796]
• Maezawa, I et al. Neurotoxicity from innate immune response is greatest with targeted replcament of e4 allele of apolipoprotein E gene is mediated by microglial p38MAPK. FASEB J. 2006-01-01 [PMID: 16481366]

Bioinformatics

• Gene Symbol: DBN1
• Entrez:
  • 396496(Chicken)
  • 1627(Human)
  • 56320(Mouse)
  • 81653(Rat)
• Uniprot:
  • P18302(Chicken)