Drebrin 1 Antibody (DBN1/2880) - Azide and BSA Free

Western Blot: Drebrin 1 Antibody (DBN1/2880) - Azide and BSA Free [NBP2-79902] - Western Blot Analysis of PC3 cell lysate using Drebrin-1 Mouse Monoclonal Antibody (DBN1/2880).

Immunohistochemistry-Paraffin: Drebrin 1 Antibody (DBN1/2880) - Azide and BSA Free [NBP2-79902] - Formalin-fixed, paraffin-embedded human Cervix stained with Drebrin-1 Mouse Monoclonal Antibody (DBN1/2880).

Immunohistochemistry-Paraffin: Drebrin 1 Antibody (DBN1/2880) - Azide and BSA Free [NBP2-79902] - Formalin-fixed, paraffin-embedded human Brain stained with Drebrin-1 Mouse Monoclonal Antibody (DBN1/2880).

Protein Array: Drebrin 1 Antibody (DBN1/2880) - Azide and BSA Free [NBP2-79902] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Drebrin 1 Antibody (DBN1/2880). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

• Reactivity: Hu
• Applications: WB, IHC, MA
• Clone: DBN1/2880
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Drebrin 1 Antibody (DBN1/2880) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79767).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant fragment (aa 150-281) of human Drebrin 1 protein (exact sequence is proprietary) (Uniprot: Q16643)
• Localization: Cytoplasmic
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DBN1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
  • Western Blot
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 120 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Drebrin 1 Antibody (DBN1/2880) - Azide and BSA Free

• D0S117E
• DBN1
• Developmentally-regulated brain protein
• DKFZp434D064
• Drba
• Drebrin 1
• Drebrin A
• Drebrin E
• Drebrin E2
• drebrin
• drebrin-1

Background

Drebrins are actin-binding proteins, that are developmentally regulated in the process of neuronal growth. Three isoforms, E1 and E2 (embryonic) and A (adult) are generated by alternative RNA splicing from a single debrin gene in the chicken brain. The expression pattern of each isoform is regulated spatially and temporally in the developing brain. It has been suggested that debrin is one of the intracellular regulators of the neuronal morphogenesis (4). Debrin has also been detected in other kinds of non-neuronal human and bovine tissue but its exact role is unknown.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DBN1