DRAK2 Antibody

Product Discontinued

DRAK2 Antibody Summary

• Immunogen: Peptide with sequence C-HSVRSSEDKTSK corresponding to internal region according to NP_004217.1.
• Epitope: C-HSVRSSEDKTSK
• Specificity: This antibody may cross-react with rat STK17B (GeneID: 170904).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: STK17B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 45 kDa band observed in MOLT4 lysates (calculated MW of 42.3 kDa band according to NP_004217.1).

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DRAK2 Antibody

• DAP kinase-related apoptosis-inducing protein kinase 2
• DRAK2
• DRAK2death-associated protein kinase-related 2
• EC 2.7.11
• EC 2.7.11.1
• serine/threonine kinase 17b (apoptosis-inducing)
• serine/threonine kinase 17b
• serine/threonine-protein kinase 17B
• STK17B

Background

Protein kinases play important roles in the signal transduction in response to a variety of external stimuli. Recently, several protein kinases have been identified that may also be involved in apoptotic process. Overexpression of a serine/threonine kinase, ZIP kinase can cause the morphological changes typical of apoptosis in NIH 3T3 cells (1). Sanjo et al, have identified two new protein kinases DRAK1 and DRAK2 (Death Receptor Associated Kinase 1 and 2) (1). Both DRAKs and ZIP kinase share significant homology at the amino acid level (1,2). The kinase domains of both DRAKs, ZIP kinase are homologous to DAP (Death-associated protein) kinase, which is involved in the apoptotic signaling induced by interferon-g. Overexpression of DRAKs in NIH 3T3 cells lead to apoptosis. These transfection experiments suggest that C-terminal domain of DRAKs are important for induction of apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DRAK2 Antibody (NBP1-51948)(1)

Publication using NBP1-51948

• Friedrich ML, Cui M, Hernandez JB et al. Modulation of DRAK2 autophosphorylation by antigen receptor signaling in primary lymphocytes. J Biol Chem 2007-02-01 [PMID: 17182616]

Bioinformatics

• Gene Symbol: STK17B
• Entrez:
  • 9262(Human)
• Uniprot:
  • NP_004217.1(Human)