DPYD Antibody

DPYD antibody [N1N2], N-term detects DPYD protein at cytoplasm on H358 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded H358 xenograft. DPYD antibody [N1N2], N-term (NBP3-48502) dilution: 1:500. Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min

Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DPYD antibody [N1N2], N-term (NBP3-48502) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DPYD antibody [N1N2], N-term (NBP3-48502) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

DPYD Antibody Summary

• Description: Novus Biologicals Rabbit DPYD Antibody (NBP3-48502) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human DPYD. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DPYD
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for DPYD Antibody

• DHP
• DHPDHASE
• dihydropyrimidine dehydrogenase [NADP+]
• dihydropyrimidine dehydrogenase
• Dihydrothymine dehydrogenase
• Dihydrouracil dehydrogenase
• DPDMGC132008
• EC 1.3.1.2
• MGC70799

Background

DPYD is encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. Mutations in this gene result in dihydropyrimidine dehydrogenase deficiency, an error in pyrimidine metabolism associated with thymine-uraciluria and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DPYD