DPPIV/CD26 Antibody [Unconjugated]

DPPIV/CD26 was detected in immersion fixed frozen sections of mouse thymus using Goat Anti-Mouse DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF954) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western blot shows lysates of mouse thymus tissue and rat lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF954) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for DPPIV/CD26 at approximately 100-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of rat lung tissue and mouse thymus tissue, loaded at 0.2 mg/mL. A specific band was detected for DPPIV/CD26 at approximately 144-169 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF954) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Mu
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

DPPIV/CD26 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse DPPIV/CD26
  Ser29-His760
  Accession # P28843
• Specificity: Detects mouse DPPIV/CD26 in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross‑reactivity with recombinant human DPPIV/CD26 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DPP4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot .25 ug/mL
  • Simple Western 5 ug/mL
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DPPIV/CD26 Antibody [Unconjugated]

• ADABP
• ADCP-2
• ADCP2DPP IV
• Adenosine deaminase complexing protein 2TP103
• CD26 antigen
• CD26
• CD26T-cell activation antigen CD26
• dipeptidyl peptidase 4
• Dipeptidyl peptidase IV
• dipeptidylpeptidase 4
• dipeptidyl-peptidase 4
• dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2)
• DPP4
• DPPIV
• EC 3.4.14.5

Background

DPPIV/CD26 (EC 3.4.14.5) is a serine exopeptidase that releases Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides (1, 2). It is a type II membrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a long extracellular domain (3‑5). The extracellular domain contains glycosylation sites, a cysteine-rich region and the catalytic active site (Ser, Asp and His charge relay system). The amino acid sequence of the mouse DPPIV/CD26 extracellular domain is 84% and 91% identical to the human and rat counterparts, respectively. In the native state, DPPIV/CD26 is present as a noncovalently linked homodimer on the cell surface of a variety of cell types. The soluble form is also detectable in human serum and other body fluids, the levels of which may have clinical significance in patients with cancer, liver and kidney diseases, and depression.

DPPIV/CD26 plays an important role in many biological and pathological processes. It functions as T cell-activating molecule (THAM). It serves as a co‑factor for entry of HIV in CD4⁺ cells (6). It binds adenosine deaminase, the deficiency of which causes severe combined immunodeficiency disease in humans (7). It cleaves chemokines such as stromal-cell-derived factor 1 alpha and macrophage-derived chemokine (8, 9). It degrades peptide hormones such as glucagon (10). It truncates procalcitonin, a marker for systemic bacterial infections with elevated levels detected in patients with thermal injury, sepsis and severe infection, and in children with bacterial meningitis (11).

1. Misumi, Y. and Y. Ikehara (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (eds), p. 1905, Elsevier, London.
2. Ikehara, Y. et al. (1994) Methods Enzymol. 244:215.
3. Marguet, D. et al. (1992) J. Biol. Chem. 267:2200.
4. Bernard, A.M. et al. (1994) Biochemistry 33:15204.
5. Vivier, I. et al. (1991) J. Immunol. 147:447.
6. Callebaut, C. et al. (1993) Science 262:2045.
7. Kameoka, J. et al. (1993) Science 261:466.
8. Ohtsuki, T. et al. (1998) FEBS Lett. 431:236.
9. Proost, P. et al. (1999) J. Biol. Chem. 274:3988.
10. Hinke, S.A. et al. (2000) J. Biol. Chem. 275:3827.
11. Wrenger, S. et al. (2000) FEBS Lett. 466:155.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DPPIV/CD26 Antibody (AF954)(8)

Publications using AF954

• Y Takagaki, S Shi, M Katoh, M Kitada, K Kanasaki, D Koya Dipeptidyl peptidase-4 plays a pathogenic role in BSA-induced kidney injury in diabetic mice Sci Rep, 2019;9(1):7519. 2019 [PMID: 31101909] (IHC-P, Mouse)
• DS Ghorpade, L Ozcan, Z Zheng, SM Nicoloro, Y Shen, E Chen, M Blüher, MP Czech, I Tabas Hepatocyte-secreted DPP4 in obesity promotes adipose inflammation and insulin resistance Nature, 2018;555(7698):673-677. 2018 [PMID: 29562231] (Immunodepletion, Mouse)
• Effect of Antifibrotic MicroRNAs Crosstalk on the Action of N-acetyl-seryl-aspartyl-lysyl-proline in Diabetes-related Kidney Fibrosis Sci Rep, 2016;6(0):29884. 2016 [PMID: 27425816] (IHC, Mouse)
• Chen Y, Chen H, Chien C, Wu S, Ho Y, Yu C, Chang M Contribution of Mature Hepatocytes to Biliary Regeneration in Rats with Acute and Chronic Biliary Injury. PLoS ONE, 2015;10(8):e0134327. 2015 [PMID: 26308208] (IHC, Rat)
• Kanasaki K, Shi S, Kanasaki M, He J, Nagai T, Nakamura Y, Ishigaki Y, Kitada M, Srivastava S, Koya D Linagliptin-mediated DPP-4 inhibition ameliorates kidney fibrosis in streptozotocin-induced diabetic mice by inhibiting endothelial-to-mesenchymal transition in a therapeutic regimen. Diabetes, 2014;63(6):2120-31. 2014 [PMID: 24574044] (IHC, Mouse)
• Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F Distinct fibroblast lineages determine dermal architecture in skin development and repair. Nature, 2013;504(7479):277-81. 2013 [PMID: 24336287] (IHC-Fr, Mouse)
• Goldman O, Han S, Sourisseau M, Dziedzic N, Hamou W, Corneo B, D'Souza S, Sato T, Kotton D, Bissig K, Kalir T, Jacobs A, Evans T, Evans M, Gouon-Evans V KDR identifies a conserved human and murine hepatic progenitor and instructs early liver development. Cell Stem Cell, 2013;12(6):748-60. 2013 [PMID: 23746980] (IHC, Mouse)
• Takasawa W, Ohnuma K, Hatano R, Endo Y, Dang NH, Morimoto C Inhibition of dipeptidyl peptidase 4 regulates microvascular endothelial growth induced by inflammatory cytokines. Biochem. Biophys. Res. Commun., 2010;401(1):7-12. 2010 [PMID: 20828536] (ICC, Mouse)

Bioinformatics

• Gene Symbol: DPP4
• Uniprot:
  • P28843()