DPPIV/CD26 Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse thymus tissue and rat lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF954) followed by ...read more
DPPIV/CD26 was detected in immersion fixed frozen sections of mouse thymus using Goat Anti-Mouse DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF954) at 1.7 µg/mL overnight at 4 °C. Tissue was ...read more
Simple Western lane view shows lysates of rat lung tissue and mouse thymus tissue, loaded at 0.2 mg/mL. A specific band was detected for DPPIV/CD26 at approximately 144-169 kDa (as indicated) using 5 µg/mL of ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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DPPIV/CD26 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse DPPIV/CD26
Ser29-His760
Accession # P28843
Specificity
Detects mouse DPPIV/CD26 in direct ELISAs and Western blots. In direct ELISAs, less than  20% cross‑reactivity with recombinant human DPPIV/CD26 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DPP4
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot .25 ug/mL
Publications
Read Publications using
AF954 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DPPIV/CD26 Antibody [Unconjugated]

  • ADABP
  • ADCP-2
  • ADCP2DPP IV
  • Adenosine deaminase complexing protein 2TP103
  • CD26 antigen
  • CD26
  • CD26T-cell activation antigen CD26
  • dipeptidyl peptidase 4
  • Dipeptidyl peptidase IV
  • dipeptidylpeptidase 4
  • dipeptidyl-peptidase 4
  • dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2)
  • DPP4
  • DPPIV
  • EC 3.4.14.5

Background

DPPIV/CD26 (EC 3.4.14.5) is a serine exopeptidase that releases Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides (1, 2). It is a type II membrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a long extracellular domain (3‑5). The extracellular domain contains glycosylation sites, a cysteine-rich region and the catalytic active site (Ser, Asp and His charge relay system). The amino acid sequence of the mouse DPPIV/CD26 extracellular domain is 84% and 91% identical to the human and rat counterparts, respectively. In the native state, DPPIV/CD26 is present as a noncovalently linked homodimer on the cell surface of a variety of cell types. The soluble form is also detectable in human serum and other body fluids, the levels of which may have clinical significance in patients with cancer, liver and kidney diseases, and depression.

DPPIV/CD26 plays an important role in many biological and pathological processes. It functions as T cell-activating molecule (THAM). It serves as a co‑factor for entry of HIV in CD4+ cells (6). It binds adenosine deaminase, the deficiency of which causes severe combined immunodeficiency disease in humans (7). It cleaves chemokines such as stromal-cell-derived factor 1 alpha and macrophage-derived chemokine (8, 9). It degrades peptide hormones such as glucagon (10). It truncates procalcitonin, a marker for systemic bacterial infections with elevated levels detected in patients with thermal injury, sepsis and severe infection, and in children with bacterial meningitis (11).

  1. Misumi, Y. and Y. Ikehara (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (eds), p. 1905, Elsevier, London.
  2. Ikehara, Y. et al. (1994) Methods Enzymol. 244:215.
  3. Marguet, D. et al. (1992) J. Biol. Chem. 267:2200.
  4. Bernard, A.M. et al. (1994) Biochemistry 33:15204.
  5. Vivier, I. et al. (1991) J. Immunol. 147:447.
  6. Callebaut, C. et al. (1993) Science 262:2045.
  7. Kameoka, J. et al. (1993) Science 261:466.
  8. Ohtsuki, T. et al. (1998) FEBS Lett. 431:236.
  9. Proost, P. et al. (1999) J. Biol. Chem. 274:3988.
  10. Hinke, S.A. et al. (2000) J. Biol. Chem. 275:3827.
  11. Wrenger, S. et al. (2000) FEBS Lett. 466:155.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DPPIV/CD26 Antibody (AF954)(36)

We have publications tested in 3 confirmed species: Mouse, Rat, Transgenic Mouse.

We have publications tested in 7 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Immunodepletion, Western Blot.


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Flow Cytometry
(1)
ICC
(4)
IHC
(13)
IHC-Fr
(1)
IHC-P
(1)
Immunodepletion
(1)
Western Blot
(1)
All Applications
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Mouse
(17)
Rat
(1)
Transgenic Mouse
(2)
All Species
Showing Publications 1 - 10 of 36. Show All 36 Publications.
Publications using AF954 Applications Species
Plá, V;Bitsika, S;Giannetto, M;Ladron-de-Guevara, A;Gahn-Martinez, D;Mori, Y;Nedergaard, M;Møllgård, K; Structural characterization of SLYM - a 4 th meningeal membrane bioRxiv : the preprint server for biology 2023-10-28 [PMID: 37961461] (IHC, Mouse, Transgenic Mouse) IHC Mouse, Transgenic Mouse
Sun, L;Zhang, X;Wu, S;Liu, Y;Guerrero-Juarez, CF;Liu, W;Huang, J;Yao, Q;Yin, M;Li, J;Ramos, R;Liao, Y;Wu, R;Xia, T;Zhang, X;Yang, Y;Li, F;Heng, S;Zhang, W;Yang, M;Tzeng, CM;Ji, C;Plikus, MV;Gallo, RL;Zhang, LJ; Dynamic interplay between IL-1 and WNT pathways in regulating dermal adipocyte lineage cells during skin development and wound regeneration Cell reports 2023-06-16 [PMID: 37330908]
Ari Matsumoto, Miki Hiroi, Kazumasa Mori, Nobuharu Yamamoto, Yoshihiro Ohmori Differential Anti-Tumor Effects of IFN-Inducible Chemokines CXCL9, CXCL10, and CXCL11 on a Mouse Squamous Cell Carcinoma Cell Line Medical Sciences 4/25/2023 [PMID: 37218983]
Evgenia Fadzeyeva, Cassandra A.A. Locatelli, Natasha A. Trzaskalski, My-Anh Nguyen, Megan E. Capozzi, Branka Vulesevic et al. Pancreas-derived DPP4 is not essential for glucose homeostasis under metabolic stress iScience 5/19/2023 [PMID: 37216093]
Q Liu, C Li, B Deng, P Gao, L Wang, Y Li, M Shiri, F Alkaifi, J Zhao, JM Stephens, CA Simintiras, J Francis, J Sun, X Fu Tcf21 marks visceral adipose mesenchymal progenitors and functions as a rate-limiting factor during visceral adipose tissue development Cell Reports, 2023-02-28;42(3):112166. 2023-02-28 [PMID: 36857185] (ICC, IHC, Mouse) ICC, IHC Mouse
N Mahmud, C Eisner, S Purushotha, MA Storer, DR Kaplan, FD Miller Nail-associated mesenchymal cells contribute to and are essential for dorsal digit tip regeneration Cell Reports, 2022-12-20;41(12):111853. 2022-12-20 [PMID: 36543145] (Western Blot, Mouse) Western Blot Mouse
Jung Eun Baik, Hyeung Ju Park, Raghu P. Kataru, Ira L. Savetsky, Catherine L. Ly, Jinyeon Shin et al. TGF‐ beta 1 mediates pathologic changes of secondary lymphedema by promoting fibrosis and inflammation Clinical and Translational Medicine 6/2/2022 [PMID: 35652284]
S Pantasis, J Friemel, SM Brütsch, Z Hu, S Krautbauer, G Liebisch, J Dengjel, A Weber, S Werner, MR Bordoli Vertebrate lonesome kinase modulates the hepatocyte secretome to prevent perivascular liver fibrosis and inflammation Journal of Cell Science, 2022-04-12;0(0):. 2022-04-12 [PMID: 35293576] (IHC, Mouse) IHC Mouse
MM Capeling, S Huang, CJ Childs, JH Wu, YH Tsai, A Wu, N Garg, EM Holloway, N Sundaram, C Bouffi, M Helmrath, JR Spence Suspension culture promotes serosal mesothelial development in human intestinal organoids Cell Reports, 2022-02-15;38(7):110379. 2022-02-15 [PMID: 35172130] (IHC, Mouse) IHC Mouse
M Stefkovich, S Traynor, L Cheng, D Merrick, P Seale Dpp4+ interstitial progenitor cells contribute to basal and high fat diet-induced adipogenesis Molecular Metabolism, 2021-10-15;0(0):101357. 2021-10-15 [PMID: 34662714] (IHC, Mouse) IHC Mouse
Show All 36 Publications.

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Bioinformatics

Gene Symbol DPP4
Uniprot