DPPIV/CD26 Antibody [Unconjugated]


Western blot shows lysates of mouse thymus tissue and rat lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF954) followed by ...read more
DPPIV/CD26 was detected in immersion fixed frozen sections of mouse thymus using Goat Anti-Mouse DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF954) at 1.7 µg/mL overnight at 4 °C. Tissue was ...read more
Simple Western lane view shows lysates of rat lung tissue and mouse thymus tissue, loaded at 0.2 mg/mL. A specific band was detected for DPPIV/CD26 at approximately 144-169 kDa (as indicated) using 5 µg/mL of ...read more

Product Details

Reactivity MuSpecies Glossary
Applications WB, Simple Western, IHC

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

DPPIV/CD26 Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant mouse DPPIV/CD26
Accession # P28843
Detects mouse DPPIV/CD26 in direct ELISAs and Western blots. In direct ELISAs, less than  20% cross‑reactivity with recombinant human DPPIV/CD26 is observed.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot .25 ug/mL
Read Publications using
AF954 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DPPIV/CD26 Antibody [Unconjugated]

  • ADCP-2
  • Adenosine deaminase complexing protein 2TP103
  • CD26 antigen
  • CD26
  • CD26T-cell activation antigen CD26
  • dipeptidyl peptidase 4
  • Dipeptidyl peptidase IV
  • dipeptidylpeptidase 4
  • dipeptidyl-peptidase 4
  • dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2)
  • DPP4
  • EC


DPPIV/CD26 (EC is a serine exopeptidase that releases Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides (1, 2). It is a type II membrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a long extracellular domain (3‑5). The extracellular domain contains glycosylation sites, a cysteine-rich region and the catalytic active site (Ser, Asp and His charge relay system). The amino acid sequence of the mouse DPPIV/CD26 extracellular domain is 84% and 91% identical to the human and rat counterparts, respectively. In the native state, DPPIV/CD26 is present as a noncovalently linked homodimer on the cell surface of a variety of cell types. The soluble form is also detectable in human serum and other body fluids, the levels of which may have clinical significance in patients with cancer, liver and kidney diseases, and depression.

DPPIV/CD26 plays an important role in many biological and pathological processes. It functions as T cell-activating molecule (THAM). It serves as a co‑factor for entry of HIV in CD4+ cells (6). It binds adenosine deaminase, the deficiency of which causes severe combined immunodeficiency disease in humans (7). It cleaves chemokines such as stromal-cell-derived factor 1 alpha and macrophage-derived chemokine (8, 9). It degrades peptide hormones such as glucagon (10). It truncates procalcitonin, a marker for systemic bacterial infections with elevated levels detected in patients with thermal injury, sepsis and severe infection, and in children with bacterial meningitis (11).

  1. Misumi, Y. and Y. Ikehara (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (eds), p. 1905, Elsevier, London.
  2. Ikehara, Y. et al. (1994) Methods Enzymol. 244:215.
  3. Marguet, D. et al. (1992) J. Biol. Chem. 267:2200.
  4. Bernard, A.M. et al. (1994) Biochemistry 33:15204.
  5. Vivier, I. et al. (1991) J. Immunol. 147:447.
  6. Callebaut, C. et al. (1993) Science 262:2045.
  7. Kameoka, J. et al. (1993) Science 261:466.
  8. Ohtsuki, T. et al. (1998) FEBS Lett. 431:236.
  9. Proost, P. et al. (1999) J. Biol. Chem. 274:3988.
  10. Hinke, S.A. et al. (2000) J. Biol. Chem. 275:3827.
  11. Wrenger, S. et al. (2000) FEBS Lett. 466:155.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DPPIV/CD26 Antibody (AF954)(36)

We have publications tested in 3 confirmed species: Mouse, Rat, Transgenic Mouse.

We have publications tested in 7 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Immunodepletion, Western Blot.

Filter By Application
Flow Cytometry
Western Blot
All Applications
Filter By Species
Transgenic Mouse
All Species
Showing Publications 1 - 10 of 36. Show All 36 Publications.
Publications using AF954 Applications Species
Plá, V;Bitsika, S;Giannetto, M;Ladron-de-Guevara, A;Gahn-Martinez, D;Mori, Y;Nedergaard, M;Møllgård, K; Structural characterization of SLYM - a 4 th meningeal membrane bioRxiv : the preprint server for biology 2023-10-28 [PMID: 37961461] (IHC, Mouse, Transgenic Mouse) IHC Mouse, Transgenic Mouse
Sun, L;Zhang, X;Wu, S;Liu, Y;Guerrero-Juarez, CF;Liu, W;Huang, J;Yao, Q;Yin, M;Li, J;Ramos, R;Liao, Y;Wu, R;Xia, T;Zhang, X;Yang, Y;Li, F;Heng, S;Zhang, W;Yang, M;Tzeng, CM;Ji, C;Plikus, MV;Gallo, RL;Zhang, LJ; Dynamic interplay between IL-1 and WNT pathways in regulating dermal adipocyte lineage cells during skin development and wound regeneration Cell reports 2023-06-16 [PMID: 37330908]
Ari Matsumoto, Miki Hiroi, Kazumasa Mori, Nobuharu Yamamoto, Yoshihiro Ohmori Differential Anti-Tumor Effects of IFN-Inducible Chemokines CXCL9, CXCL10, and CXCL11 on a Mouse Squamous Cell Carcinoma Cell Line Medical Sciences 4/25/2023 [PMID: 37218983]
Evgenia Fadzeyeva, Cassandra A.A. Locatelli, Natasha A. Trzaskalski, My-Anh Nguyen, Megan E. Capozzi, Branka Vulesevic et al. Pancreas-derived DPP4 is not essential for glucose homeostasis under metabolic stress iScience 5/19/2023 [PMID: 37216093]
Q Liu, C Li, B Deng, P Gao, L Wang, Y Li, M Shiri, F Alkaifi, J Zhao, JM Stephens, CA Simintiras, J Francis, J Sun, X Fu Tcf21 marks visceral adipose mesenchymal progenitors and functions as a rate-limiting factor during visceral adipose tissue development Cell Reports, 2023-02-28;42(3):112166. 2023-02-28 [PMID: 36857185] (ICC, IHC, Mouse) ICC, IHC Mouse
N Mahmud, C Eisner, S Purushotha, MA Storer, DR Kaplan, FD Miller Nail-associated mesenchymal cells contribute to and are essential for dorsal digit tip regeneration Cell Reports, 2022-12-20;41(12):111853. 2022-12-20 [PMID: 36543145] (Western Blot, Mouse) Western Blot Mouse
Jung Eun Baik, Hyeung Ju Park, Raghu P. Kataru, Ira L. Savetsky, Catherine L. Ly, Jinyeon Shin et al. TGF‐ beta 1 mediates pathologic changes of secondary lymphedema by promoting fibrosis and inflammation Clinical and Translational Medicine 6/2/2022 [PMID: 35652284]
S Pantasis, J Friemel, SM Brütsch, Z Hu, S Krautbauer, G Liebisch, J Dengjel, A Weber, S Werner, MR Bordoli Vertebrate lonesome kinase modulates the hepatocyte secretome to prevent perivascular liver fibrosis and inflammation Journal of Cell Science, 2022-04-12;0(0):. 2022-04-12 [PMID: 35293576] (IHC, Mouse) IHC Mouse
MM Capeling, S Huang, CJ Childs, JH Wu, YH Tsai, A Wu, N Garg, EM Holloway, N Sundaram, C Bouffi, M Helmrath, JR Spence Suspension culture promotes serosal mesothelial development in human intestinal organoids Cell Reports, 2022-02-15;38(7):110379. 2022-02-15 [PMID: 35172130] (IHC, Mouse) IHC Mouse
M Stefkovich, S Traynor, L Cheng, D Merrick, P Seale Dpp4+ interstitial progenitor cells contribute to basal and high fat diet-induced adipogenesis Molecular Metabolism, 2021-10-15;0(0):101357. 2021-10-15 [PMID: 34662714] (IHC, Mouse) IHC Mouse
Show All 36 Publications.

Reviews for DPPIV/CD26 Antibody (AF954) (0)

There are no reviews for DPPIV/CD26 Antibody (AF954). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DPPIV/CD26 Antibody (AF954) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our DPPIV/CD26 Antibody [Unconjugated] and receive a gift card or discount.


Gene Symbol DPP4