DPPIV/CD26 Antibody (JM11-42)

Western Blot: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Western blot analysis of DPPIV/CD26 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse lung tissue lysate Lane 2: mouse heart tissue lysate Lane 3: human kidney tissue lysate Lane 4: Raji cell lysate Lane 5: PC-3 cell lysate

Immunocytochemistry/Immunofluorescence: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Staining CD26 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Analysis of paraffin-embedded human liver tissue using anti-CD26 antibody. Counter stained with hematoxylin.

Flow Cytometry: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Analysis of Hela cells with CD26 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Staining CD26 in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Analysis of paraffin-embedded rat epididymis tissue using anti-CD26 antibody. Counter stained with hematoxylin.

Western Blot: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - All lanes: Western blot analysis of CD26 with anti-CD26 antibody at 1:500 dilution.Lane 1: Wild-type Hela whole cell lysate (10 ug). Lane 2: CD26 knockout Hela whole cell lysate (10 ug).NBP2-78791 was shown to specifically react with CD26 in wild-type Hela cells. NO band was observed when CD26 knockout sample was tested. Wild-type and CD26 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, KO
• Clone: JM11-42
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

DPPIV/CD26 Antibody (JM11-42) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within Human DPPIV/CD26 aa 701-747 / 766 (Extracellular). (SwissProt: P27487 Human; SwissProt: P28843 Mouse; SwissProt: P14740 Rat)
• Localization: Secreted, Cell membrane, Apical cell membrane, invadopodium membrane, lamellipodium membrane, Cell junction, Membrane raft.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DPP4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Knockout Validated
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for DPPIV/CD26 Antibody (JM11-42)

• ADABP
• ADCP-2
• ADCP2DPP IV
• Adenosine deaminase complexing protein 2TP103
• CD26 antigen
• CD26
• CD26T-cell activation antigen CD26
• dipeptidyl peptidase 4
• Dipeptidyl peptidase IV
• dipeptidylpeptidase 4
• dipeptidyl-peptidase 4
• dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2)
• DPP4
• DPPIV
• EC 3.4.14.5

Background

CD26 is encoded by this gene is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DPP4