DPM1 Overexpression Lysate (Native)

Images

 
Western Blot: DPM1 Overexpression Lysate (Adult Normal) [NBL1-09992] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DPM1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

DPM1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC202817 and based on accession number NM_003859. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit (DPM1), mRNA.
Gene
DPM1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
29.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for DPM1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for DPM1 Overexpression Lysate (Native)

  • CDGIEEC 2.4.1.83
  • dolichol monophosphate mannose synthase
  • dolichol-phosphate mannosyltransferase
  • Dolichyl-phosphate beta-D-mannosyltransferase
  • dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit
  • DPM synthase
  • Mannose-P-dolichol synthase
  • MPD synthase
  • MPDSDolichol-phosphate mannose synthase

Background

Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-38056
Species: Hu
Applications: IHC, IHC-P
NBP1-81216
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-24557
Species: Hu, Eq, Pm
Applications: WB, IHC-P
NBP2-02541
Species: Hu, Pm
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-81838
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-84570
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-87692
Species: Hu, Mu, Rt, Ha
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-32539
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-30375
Species: Hu
Applications: IHC, IHC-P
NBP2-14246
Species: Hu
Applications: IHC, IHC-P
NB500-330
Species: Hu
Applications: B/N, Flow, IHC, IHC-P, IP, CyTOF-ready
NBP1-62494
Species: Hu
Applications: WB
46780002
Species: Hu
Applications: ELISA
H00022845-G01-10ug
Species: Hu
NBP2-13760
Species: Hu
Applications: IHC, IHC-P
NBP1-47914
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-85699
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-82845
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Publications for DPM1 Lysate (NBL1-09992) (0)

There are no publications for DPM1 Lysate (NBL1-09992).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for DPM1 Lysate (NBL1-09992) (0)

There are no reviews for DPM1 Lysate (NBL1-09992). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DPM1 Lysate (NBL1-09992) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional DPM1 Products

Bioinformatics Tool for DPM1 Lysate (NBL1-09992)

Discover related pathways, diseases and genes to DPM1 Lysate (NBL1-09992). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for DPM1 Lysate (NBL1-09992)

Discover more about diseases related to DPM1 Lysate (NBL1-09992).
 

Pathways for DPM1 Lysate (NBL1-09992)

View related products by pathway.

PTMs for DPM1 Lysate (NBL1-09992)

Learn more about PTMs related to DPM1 Lysate (NBL1-09992).

Blogs on DPM1

There are no specific blogs for DPM1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

DPM1 Antibody
NBP1-05034
Emerin Antibody
NBP1-87692

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our DPM1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol DPM1