DPM1 Antibody

Western Blot: DPM1 Antibody [NBP1-05033] - Analysis of DPM1 (mouse) in Rat Liver lysate using this antibody at 0.05 ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Rt, Mu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DPM1 Antibody Summary

• Immunogen: Peptide with sequence PQGRSSRQDKYS-C corresponding to N-Terminus according to Uniprot Mouse NP_034202.1.
• Epitope: N-Terminus
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DPM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 29 kDa band observed in rat liver lysates (calculated MW of 29.2 kDa band according to mouse NP_034202.1 and to rat NP_001100014.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DPM1 Antibody

• CDGIEEC 2.4.1.83
• dolichol monophosphate mannose synthase
• dolichol-phosphate mannosyltransferase
• Dolichyl-phosphate beta-D-mannosyltransferase
• dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit
• DPM synthase
• Mannose-P-dolichol synthase
• MPD synthase
• MPDSDolichol-phosphate mannose synthase

Background

Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DPM1 Antibody (NBP1-05033)(1)

Publication using NBP1-05033

• Ashida H, Maeda Y, Kinoshita T. DPM1, the catalytic subunit of dolichol-phosphate mannose synthase, is tethered to and stabilized on the endoplasmic reticulum membrane by DPM3 J Biol Chem 2006-01-13 [PMID: 16280320]

Bioinformatics

• Gene Symbol: DPM1
• Entrez:
  • 13480(Mouse)
  • 296394(Rat)
• Uniprot:
  • NP_034202.1(Mouse)