DPH5 Antibody - Azide and BSA Free

Western Blot: DPH5 Antibody [H00051611-B01P] - Analysis of DPH5 expression in transfected 293T cell line by DPH5 polyclonal antibody. Lane 1: DPH5 transfected lysate(31.35 KDa). Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

DPH5 Antibody - Azide and BSA Free Summary

• Immunogen: DPH5 (NP_001070862.1, 1 a.a. - 285 a.a.) full-length human protein. MLYLIGLGLGDAKDITVKGLEVVRRCSRVYLEAYTSVLTVGKEALEEFYGRKLVVADREEVEQEADNILKDADISDVAFLVVGDPFGATTHSDLVLRATKLGIPYRVIHNASIMNAVGCCGLQLYKFGETVSIVFWTDTWRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLIKGRKIYEPPRYMSVNQAAQQLLEIVQNQRIRGEEPAVTEETLCVGLARVGADDQKIAAGTLRQMCTVDLGEPLHSLIITGGSIHPMEMEMLSLFSIPENSSESQSINGL
• Specificity: DPH5 - DPH5 homolog (S. cerevisiae),
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: DPH5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DPH5 Antibody - Azide and BSA Free

• CGI-30
• Diphthamide biosynthesis methyltransferase
• diphthine synthase
• DPH5 homolog (S. cerevisiae)
• EC 2.1.1.98
• HSPC143
• MGC61450
• NPD015
• protein x 0011

Background

This gene encodes a component of the diphthamide synthesis pathway. Diphthamide is a post-translationally modified histidine residue found only on translation elongation factor 2. It is conserved from archaebacteria to humans, and is targeted by diphtheria toxin and Pseudomonas exotoxin A to halt cellular protein synthesis. The yeast and Chinese hamster homologs of this protein catalyze the trimethylation of the histidine residue on elongation factor 2, resulting in a diphthine moiety that is subsequently amidated to yield diphthamide. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DPH5
• Entrez:
  • 51611(Human)
• Uniprot:
  • NP_001070862.1(Human)