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Dopamine D5R/DRD5 Antibody (SG4-D1b)

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Western Blot: Dopamine Receptor D5 Antibody (SG4-D1b) [NB110-60019] - Detection of Dopamine Receptor D1a of Sf9 cells transfected with rat D1b. Lane 1: Sf9-D1b lysate Lane 2: Sf9-D1a lyate (Please note that these results are similiar to those shown in J. Neuroimmunol. 101:170-187 publication.)
Western Blot: Dopamine Receptor D5 Antibody (SG4-D1b) [NB110-60019] - Detection of Dopamine Receptor D1a of Sf9 cells transfected with rat D1b. Lane 1: Sf9-D1b lysate Lane 2: Sf9-D1a lyate (Please note that these ...read more

Product Details

Summary
Reactivity Mu, Rt, Hu(-)Species Glossary
Applications WB, ICC/IF, IHC
Clone
SG4-D1b
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB110-60019AF350
Alexa Fluor 405
NB110-60019AF405
Alexa Fluor 488
NB110-60019AF488
Alexa Fluor 532
NB110-60019AF532
Alexa Fluor 594
NB110-60019AF594
Alexa Fluor 647
NB110-60019AF647
Alexa Fluor 700
NB110-60019AF700
Alexa Fluor 750
NB110-60019AF750
Biotin
NB110-60019B
DyLight 350
NB110-60019UV
DyLight 405
NB110-60019V
DyLight 488
NB110-60019G
DyLight 550
NB110-60019R
DyLight 594
NB110-60019DL594
DyLight 650
NB110-60019C
DyLight 680
NB110-60019FR
DyLight 755
NB110-60019IR
FITC
NB110-60019F
HRP
NB110-60019H
Janelia Fluor 549
NB110-60019JF549
Janelia Fluor 646
NB110-60019JF646
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-80701
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Dopamine D5R/DRD5 Antibody (SG4-D1b) Summary

Immunogen
Recombinant rat Dopamine Receptor D5 (within the last 118 residues of the C-terminus). [Swiss-Prot# P25115]
Localization
Cell membrane; Multi-pass membrane protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DRD5
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:200-1:500
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:1000
Application Notes
This Dopamine Receptor D5 antibody is useful for Immunocytochemistry/Immunofluorescence, Western Blot and Immunohistochemistry. In Western Blot a band is seen ~52-64 kDa in SF9 cell lysate. There is no band in striatal tissue lysate. Do NOT boil samples.
Publications
Read Publication using
NB110-60019 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Rat. Reactivity with mouse has had mixed results. It does not react with human protein.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Dopamine D5R/DRD5 Antibody (SG4-D1b)

  • D(1B) dopamine receptor
  • D(5) dopamine receptor
  • D1beta dopamine receptor
  • DBDR
  • Dopamine D5 R
  • Dopamine D5 receptor
  • Dopamine D5R
  • dopamine receptor D1B
  • dopamine receptor D5
  • DRD1B
  • DRD1BDBDR
  • DRD1L2
  • DRD1L2MGC10601
  • DRD5

Background

Dopamine Receptor D5 (DRD5 or D5R/D5 receptor) belongs to GPCR1 family and represents dopamine receptor whose activity is mediated by G proteins that activate adenylyl cyclase. Localized in cell membranes as multi-pass trans-membrane protein, DRD5 exhibits widespread expression in CNS tissues- amygdala, frontal cortex, hippocampus, striatum, basal forebrain, hypothalamus, cerebellum and thalamus - whereas, at cellular level, the large aspiny neurons of neostriatum, which are typically cholinergic interneurons, only express DRD5. Besides stimulating adenyl cyclase activity, DRD5 also interacts with GABRG2 suggesting that it may modulate GABAAR-mediated activity via second messenger cascades as well as direct receptor-receptor interaction. Mice lacking DRD5 are less active in baseline locomotor exploration while their exploratory activity increases, suggesting its inhibitory effect on locomotion. DRD5 has been suggested to be implicated in regulating hypothalamic function and some forms of motor control.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Dopamine D5R/DRD5
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Dopamine D5R/DRD5 Antibody (SG4-D1b)
Species: Mu, Rt, Hu(-)
Applications: WB, ICC/IF, IHC

Publications for Dopamine D5R/DRD5 Antibody (NB110-60019)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 2 applications: IHC, WB.


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View specific protocols for Dopamine D5R/DRD5 Antibody (NB110-60019): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-60019AF350
Alexa Fluor 405 NB110-60019AF405
Alexa Fluor 488 NB110-60019AF488
Alexa Fluor 532 NB110-60019AF532
Alexa Fluor 594 NB110-60019AF594
Alexa Fluor 647 NB110-60019AF647
Alexa Fluor 700 NB110-60019AF700
Alexa Fluor 750 NB110-60019AF750
Biotin NB110-60019B
DyLight 350 NB110-60019UV
DyLight 405 NB110-60019V
DyLight 488 NB110-60019G
DyLight 550 NB110-60019R
DyLight 594 NB110-60019DL594
DyLight 650 NB110-60019C
DyLight 680 NB110-60019FR
DyLight 755 NB110-60019IR
FITC NB110-60019F
HRP NB110-60019H
Janelia Fluor 549 NB110-60019JF549
Janelia Fluor 646 NB110-60019JF646
Learn about Custom Antibody Labeling

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Diseases for Dopamine D5R/DRD5 Antibody (NB110-60019)

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Gene Symbol DRD5
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