Home » Dopamine D5R/DRD5 » Dopamine Receptor D5 Research Products » Dopamine D5R/DRD5 Antibody (SG4-D1b)

Dopamine D5R/DRD5 Antibody (SG4-D1b)

 (1 Publication)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western Blot: Dopamine Receptor D5 Antibody (SG4-D1b) [NB110-60019] - Detection of Dopamine Receptor D1a of Sf9 cells transfected with rat D1b. Lane 1: Sf9-D1b lysate Lane 2: Sf9-D1a lyate (Please note that these results are similiar to those shown in J. Neuroimmunol. 101:170-187 publication.)
Western Blot: Dopamine Receptor D5 Antibody (SG4-D1b) [NB110-60019] - Detection of Dopamine Receptor D1a of Sf9 cells transfected with rat D1b. Lane 1: Sf9-D1b lysate Lane 2: Sf9-D1a lyate (Please note that these ...read more

Product Details

Summary
Reactivity Mu, Rt, Hu(-)Species Glossary
Applications WB, ICC/IF, IHC
Clone
SG4-D1b
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB110-60019AF350
Alexa Fluor 405
NB110-60019AF405
Alexa Fluor 488
NB110-60019AF488
Alexa Fluor 532
NB110-60019AF532
Alexa Fluor 594
NB110-60019AF594
Alexa Fluor 647
NB110-60019AF647
Alexa Fluor 700
NB110-60019AF700
Alexa Fluor 750
NB110-60019AF750
Biotin
NB110-60019B
DyLight 350
NB110-60019UV
DyLight 405
NB110-60019V
DyLight 488
NB110-60019G
DyLight 550
NB110-60019R
DyLight 594
NB110-60019DL594
DyLight 650
NB110-60019C
DyLight 680
NB110-60019FR
DyLight 755
NB110-60019IR
FITC
NB110-60019F
HRP
NB110-60019H
Janelia Fluor 549
NB110-60019JF549
Janelia Fluor 646
NB110-60019JF646
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-80701
100% Guarantee on every product

Dopamine D5R/DRD5 Antibody (SG4-D1b) Summary

Immunogen
Recombinant rat Dopamine Receptor D5 (within the last 118 residues of the C-terminus). [Swiss-Prot# P25115]
Localization
Cell membrane; Multi-pass membrane protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DRD5
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:200-1:500
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:1000
Application Notes
This Dopamine Receptor D5 antibody is useful for Immunocytochemistry/Immunofluorescence, Western Blot and Immunohistochemistry. In Western Blot a band is seen ~52-64 kDa in SF9 cell lysate. There is no band in striatal tissue lysate. Do NOT boil samples.
Publications
Read Publication using
NB110-60019 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Rat. Reactivity with mouse has had mixed results. It does not react with human protein.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Dopamine D5R/DRD5 Antibody (SG4-D1b)

  • D(1B) dopamine receptor
  • D(5) dopamine receptor
  • D1beta dopamine receptor
  • DBDR
  • Dopamine D5 R
  • Dopamine D5 receptor
  • Dopamine D5R
  • dopamine receptor D1B
  • dopamine receptor D5
  • DRD1B
  • DRD1BDBDR
  • DRD1L2
  • DRD1L2MGC10601
  • DRD5

Background

Dopamine Receptor D5 (DRD5 or D5R/D5 receptor) belongs to GPCR1 family and represents dopamine receptor whose activity is mediated by G proteins that activate adenylyl cyclase. Localized in cell membranes as multi-pass trans-membrane protein, DRD5 exhibits widespread expression in CNS tissues- amygdala, frontal cortex, hippocampus, striatum, basal forebrain, hypothalamus, cerebellum and thalamus - whereas, at cellular level, the large aspiny neurons of neostriatum, which are typically cholinergic interneurons, only express DRD5. Besides stimulating adenyl cyclase activity, DRD5 also interacts with GABRG2 suggesting that it may modulate GABAAR-mediated activity via second messenger cascades as well as direct receptor-receptor interaction. Mice lacking DRD5 are less active in baseline locomotor exploration while their exploratory activity increases, suggesting its inhibitory effect on locomotion. DRD5 has been suggested to be implicated in regulating hypothalamic function and some forms of motor control.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Dopamine Receptor
D4 Antibody
NBP2-92717
Western Blot: Dopamine Receptor D4 Antibody [NBP2-92717] - Analysis of extracts of various cell lines, using Dopamine Receptor D4 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
Immunohistochemistry-Paraffin: Dopamine Receptor D4 Antibody [NBP2-92717] - Paraffin-embedded rat retina using Dopamine Receptor D4.
Species: Mu, Rt
Applications: WB, IHC, IHC-P
SLC6A3/DAT1
Antibody (mAb16)
NBP2-22164
Western Blot: SLC6A3/DAT1 Antibody (mAb16) [NBP2-22164] - Western blot analysis of DAT protein. Band shown at approx. 75 kDa. Image submitted by verified customer review.
Immunocytochemistry/Immunofluorescence: DAT1 Antibody (mAb16) [NBP2-22164] - The DAT1 antibody was tested in PC12 cells at a 1:250 dilution against DyLight 488 (Green). Actin and nuclei were counterstained with Phalloidin-AlexaFluor 568 (Red) and DAPI (Blue), respectively.
Immunohistochemistry-Paraffin: DAT1 Antibody (mAb16) [NBP2-22164] - IHC analysis of DAT1 in mouse brain using DAB with hematoxylin counterstain.
(3 Reviews)
(12 Publications)
Species: Mu, Rt, Hu(-)
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
BBS9 Antibody
NBP1-88927
Immunohistochemistry-Paraffin: BBS9 Antibody [NBP1-88927] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
(3 Publications)
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
LMOD1 Antibody
NBP1-89396
Western Blot: LMOD1 Antibody [NBP1-89396] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/Immunofluorescence: LMOD1 Antibody [NBP1-89396] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: LMOD1 Antibody [NBP1-89396] - Staining of human smooth muscle.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Dopamine D3R/DRD3
Antibody
NBP3-03577
Western Blot: Dopamine D3R/DRD3 Antibody [NBP3-03577] - Analysis of extracts of mouse brain, using Dopamine D3R/DRD3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunocytochemistry/Immunofluorescence: Dopamine D3R/DRD3 Antibody [NBP3-03577] - Analysis of U2OS cells using Dopamine D3R/DRD3 antibody. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Dopamine D3R/DRD3 Antibody [NBP3-03577] - Paraffin-embedded human breast cancer using Dopamine D3R/DRD3 antibody at dilution of 1:100 (40x lens).
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
SNAP25 Antibody
NBP1-88769
Western Blot: SNAP25 Antibody [NBP1-88769] - Analysis in mouse cerebral cortex tissue.
Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-88769] - Analysis in human cerebral cortex and testis tissues using NBP1-88769 antibody. Corresponding SNAP25 RNA-seq data are presented for the same tissues.
Western Blot: SNAP25 Antibody [NBP1-88769] - Analysis in human cell lines U2OS and MCF-7 using anti-SNAP25 antibody. Corresponding SNAP25 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
(4 Publications)
Species: Hu, Mu
Applications: WB, IHC, IHC-P
Dopamine
beta-Hydroxylase Anti
NBP1-31386
Western Blot: Dopamine beta-Hydroxylase Antibody [NBP1-31386] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Dopamine beta hydroxylase antibody and a competitor's antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP1-31386] - Paraffin-embedded RT2 xenograft; antibody dilution 1:500.
Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP1-31386] - Paraffin-embedded U87 xenograft; antibody dilution 1:500.
(4 Publications)
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Dopamine D1R/DRD1
Antibody (SG2-D1a)
NB110-60017
Western Blot: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Analysis of lysates from Sf9 cells which were transfected with rat DR-D1b (Lane 1) or DR-D1a (Lane 2) using DRD1 antibody (clone SG2-D1a). This clone detected D1a receptor only and was not cross-reactive against DR-D1b (Note: these results are similiar to those shown in J. Neuroimmunol. 101:170-187 publication.)
Immunohistochemistry: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Representative images of immunofluorescence for c-fos and dopamine receptor D1 in the NAc. Image collected and cropped by CiteAb from the following publication (nature.com/articles/ncomms11829), licensed under a CC-BY licence.
Immunohistochemistry: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Dopamine D1 R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Analysis of Dopamine Receptor D1 in rat retinal whole mounts and sections. Image from verified customer review.
(3 Reviews)
(10 Publications)
Species: Mu, Rt, Bv(-), Ca(-), Hu(-), Po(-), Rb(-), Sh(-)
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-FrFl
NF2/Merlin Antibody
NBP1-87757
Western Blot: NF2/Merlin Antibody [NBP1-87757] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/Immunofluorescence: NF2/Merlin Antibody [NBP1-87757] - Staining of human cell line U-251 MG shows localization to plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NF2/Merlin Antibody [NBP1-87757] - Staining of human fallopian tube shows moderate cytoplasmic and moderate membranous positivity in glandular cells.
(3 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP, KD
Dopamine D2R/DRD2
Antibody
NLS1403
Immunohistochemistry-Paraffin: Dopamine D2 R/DRD2 Antibody [NLS1403] - Analysis of anti-DRD2 antibody with human brain, neurons and glia at 3 ug/ml.
(2 Publications)
Species: Hu
Applications: IHC, IHC-P
COMT Antibody
NB100-41387
Western Blot: COMT Antibody [NB100-41387] - Staining of human testis lysate (35ug protein in RIPA buffer) with Goat Anti-COMT (internal) (0.03ug/ml). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: COMT Antibody [NB100-41387] - staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: COMT Antibody [NB100-41387] - (4ug/ml) staining of paraffin embedded Human Cerebellum. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
(1 Publication)
Species: Hu
Applications: WB, ELISA, EIA, IHC, IHC-P, PEP-ELISA, S-ELISA
DIO2 Antibody
NBP1-00178
Immunocytochemistry/Immunofluorescence: DIO2 Antibody [NBP1-00178] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton.Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry-Frozen: DIO2 Antibody [NBP1-00178] - IHC analysis of DIO2 in cryo-sectioned Mouse Hippocampus using NBP1-00178 at 2ug/ml. Microwaved antigen retrieval with citrate buffer pH 4.5, HRP-staining.
Flow Cytometry: DIO2 Antibody [NBP1-00178] - Analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5%Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody
(6 Publications)
Species: Hu, Mu, Sh
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, PEP-ELISA
DIO3 Antibody
NBP1-05767
Western Blot: DIO3 Antibody [NBP1-05767] - Detection of Dio3 in rat placenta.
Flow Cytometry: DIO3 Antibody [NBP1-05767] - Analysis using the Biotin conjugate of NBP1-05767. Dio3 staining of mouse hepatic stellate cell cultured for 7 days. Image from verified customer review.
Western Blot: DIO3 Antibody [NBP1-05767] - Detection of Dio3 in rabbit brain whole cell homogenate. Photo courtesy of product review by verified customer.
(2 Reviews)
(12 Publications)
Species: Hu, Mu, Rt, Ha, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Tyrosine
Hydroxylase Antibody
NB300-109
Immunohistochemistry: Tyrosine Hydroxylase Antibody [NB300-109] - Tyrosine hydroxylase immunoreactivity in the central complex and the lateral accessory lobe. A-D: Frontal sections (immunoperoxidase preparations, dorsal to the top). E: Horizontal section (immunofluorescent preparation, posterior to the top). Scale bars = 100 um. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0160531), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-109] - Dopamine neurons in the mouse substantia nigra. ICC/IF image submitted by a verified customer review.
Simple Western: Tyrosine Hydroxylase Antibody [NB300-109] - Simple Western lane view shows a specific band for Tyrosine Hydroxylase in 0.2 mg/mL of PC-12 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
(7 Reviews)
(143 Publications)
Species: Hu, Mu, Rt, Dr, I, Ma
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, Dual ISH-IHC, IHC-FrFl, IHC-WhMt, KO

SLC6A4/5-HTTLPR/Seroto ...
NB100-1892
Western Blot: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [NB100-1892] - Human Colon lysate, 35 ug protein in RIPA buffer. Antibody at 2 ug/mL. Detection by chemiluminescence.
Immunocytochemistry/Immunofluorescence: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [NB100-1892] - Analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
(1 Publication)
Species: Hu
Applications: WB, ICC/IF, PEP-ELISA
5-HT1B Antibody
NB100-56350
Western Blot: 5-HT1B Antibody [NB100-56350] - Analysis of 5-HT1B in human brain lysate with anti-5-HT1BR pcAb (NB100-56350). A protein band of approximate molecular weight of 40-41kDa was detected.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB
D4-GDI/RhoGDI2
Antibody (97A1015) - ...
NB100-56725
Western Blot: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - WB analysis: HL60 cells were treated for different time periods with 10 ng/ml of anti-Fas mAb (NB100-56725, Clone DX2). Harvested cells were lysed and resolved by SDS-PAGE and transferred onto membrane. D4-GDI/RhoGDI2 cleavage was detected.
Immunohistochemistry-Paraffin: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - analysis of D4GD1-cv in formalin-fixed, paraffin-embedded human breast tumor tissue using an isotype control (top) and this antibody.
Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - Intracellular analysis of cleaved D4-GDI in 10^6 untreated (left) and 3 hour staurosporine-treated (right) Jurkat cells using 0.5 ug of this antibody was used for this test. Green histogram represents isotype control; red represents anti-D4-GDI antibody. Image using the Alexa Fluor 647 format of this antibody.
(1 Review)
(16 Publications)
Species: Hu, Mu
Applications: WB, Flow, IHC, IHC-P, IP, Flow-IC
MAO-A Antibody
NBP2-38868
Western Blot: MAO-A Antibody [NBP2-38868] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/Immunofluorescence: MAO-A Antibody [NBP2-38868] - Staining of human cell line RT4 shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MAO-A Antibody [NBP2-38868] - Analysis in human small intestine and lymph node tissues using HPA059299 antibody. Corresponding MAOA RNA-seq data are presented for the same tissues.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for Dopamine D5R/DRD5 Antibody (NB110-60019)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 2 applications: IHC, WB.


Filter By Application
IHC
(1)
WB
(1)
All Applications
Filter By Species
Rat
(1)
All Species

Reviews for Dopamine D5R/DRD5 Antibody (NB110-60019) (0)

There are no reviews for Dopamine D5R/DRD5 Antibody (NB110-60019). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

View specific protocols for Dopamine D5R/DRD5 Antibody (NB110-60019): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Dopamine D5R/DRD5 Antibody (NB110-60019) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-60019AF350
Alexa Fluor 405 NB110-60019AF405
Alexa Fluor 488 NB110-60019AF488
Alexa Fluor 532 NB110-60019AF532
Alexa Fluor 594 NB110-60019AF594
Alexa Fluor 647 NB110-60019AF647
Alexa Fluor 700 NB110-60019AF700
Alexa Fluor 750 NB110-60019AF750
Biotin NB110-60019B
DyLight 350 NB110-60019UV
DyLight 405 NB110-60019V
DyLight 488 NB110-60019G
DyLight 550 NB110-60019R
DyLight 594 NB110-60019DL594
DyLight 650 NB110-60019C
DyLight 680 NB110-60019FR
DyLight 755 NB110-60019IR
FITC NB110-60019F
HRP NB110-60019H
Janelia Fluor 549 NB110-60019JF549
Janelia Fluor 646 NB110-60019JF646
Learn about Custom Antibody Labeling

Additional Dopamine D5R/DRD5 Products

Bioinformatics Tool for Dopamine D5R/DRD5 Antibody (NB110-60019)

Discover related pathways, diseases and genes to Dopamine D5R/DRD5 Antibody (NB110-60019). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Dopamine D5R/DRD5 Antibody (NB110-60019)

Discover more about diseases related to Dopamine D5R/DRD5 Antibody (NB110-60019).
 

Pathways for Dopamine D5R/DRD5 Antibody (NB110-60019)

View related products by pathway.

PTMs for Dopamine D5R/DRD5 Antibody (NB110-60019)

Learn more about PTMs related to Dopamine D5R/DRD5 Antibody (NB110-60019).
 

Research Areas for Dopamine D5R/DRD5 Antibody (NB110-60019)

Find related products by research area.

Blogs on Dopamine D5R/DRD5

There are no specific blogs for Dopamine D5R/DRD5, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  NB110-60019 Specific Protocol
  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Dopamine D5R/DRD5 Antibody (SG4-D1b) and receive a gift card or discount.


Bioinformatics

Gene Symbol DRD5