Dopamine D5R/DRD5 Antibody (SG4-D1b)

Western Blot: Dopamine Receptor D5 Antibody (SG4-D1b) [NB110-60019] - Detection of Dopamine Receptor D1a of Sf9 cells transfected with rat D1b. Lane 1: Sf9-D1b lysate Lane 2: Sf9-D1a lyate (Please note that these results are similiar to those shown in J. Neuroimmunol. 101:170-187 publication.)

• Reactivity: Mu, Rt, Hu(-)
• Applications: WB, ICC/IF, IHC
• Clone: SG4-D1b
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Dopamine D5R/DRD5 Antibody (SG4-D1b) Summary

• Immunogen: Recombinant rat Dopamine Receptor D5 (within the last 118 residues of the C-terminus). [Swiss-Prot# P25115]
• Localization: Cell membrane; Multi-pass membrane protein.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DRD5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:1000
  • Western Blot 1:200-1:500
• Application Notes: This Dopamine Receptor D5 antibody is useful for Immunocytochemistry/Immunofluorescence, Western Blot and Immunohistochemistry. In Western Blot a band is seen ~52-64 kDa in SF9 cell lysate. There is no band in striatal tissue lysate. Do NOT boil samples.

Reactivity Notes

Rat. Reactivity with mouse has had mixed results. It does not react with human protein.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Dopamine D5R/DRD5 Antibody (SG4-D1b)

• D(1B) dopamine receptor
• D(5) dopamine receptor
• D1beta dopamine receptor
• DBDR
• Dopamine D5 R
• Dopamine D5 receptor
• Dopamine D5R
• dopamine receptor D1B
• dopamine receptor D5
• DRD1B
• DRD1BDBDR
• DRD1L2
• DRD1L2MGC10601
• DRD5

Background

Dopamine Receptor D5 (DRD5 or D5R/D5 receptor) belongs to GPCR1 family and represents dopamine receptor whose activity is mediated by G proteins that activate adenylyl cyclase. Localized in cell membranes as multi-pass trans-membrane protein, DRD5 exhibits widespread expression in CNS tissues- amygdala, frontal cortex, hippocampus, striatum, basal forebrain, hypothalamus, cerebellum and thalamus - whereas, at cellular level, the large aspiny neurons of neostriatum, which are typically cholinergic interneurons, only express DRD5. Besides stimulating adenyl cyclase activity, DRD5 also interacts with GABRG2 suggesting that it may modulate GABAAR-mediated activity via second messenger cascades as well as direct receptor-receptor interaction. Mice lacking DRD5 are less active in baseline locomotor exploration while their exploratory activity increases, suggesting its inhibitory effect on locomotion. DRD5 has been suggested to be implicated in regulating hypothalamic function and some forms of motor control.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dopamine D5R/DRD5 Antibody (NB110-60019)(1)

Publication using NB110-60019

• Luedtke, R et al. Immunoblot and immunohistochemical comparison of murine monoclonal antibodies specific for the rat D1a and D1b dopamine receptor subtypes. J Neuroimmunol;101(2):170-87. 1999-11-15 [PMID: 10580800] (IHC, WB, Rat)

Bioinformatics

• Gene Symbol: DRD5