Dopamine D5R/DRD5 Antibody (SG4-D1b)

Images

 
Western Blot: Dopamine Receptor D5 Antibody (SG4-D1b) [NB110-60019] - Detection of Dopamine Receptor D1a of Sf9 cells transfected with rat D1b. Lane 1: Sf9-D1b lysate Lane 2: Sf9-D1a lyate (Please note that these ...read more

Product Details

Summary
Reactivity Mu, Rt, Hu(-)Species Glossary
Applications WB, ICC/IF, IHC
Clone
SG4-D1b
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Dopamine D5R/DRD5 Antibody (SG4-D1b) Summary

Immunogen
Recombinant rat Dopamine Receptor D5 (within the last 118 residues of the C-terminus). [Swiss-Prot# P25115]
Localization
Cell membrane; Multi-pass membrane protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DRD5
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:1000
  • Western Blot 1:200-1:500
Application Notes
This Dopamine Receptor D5 antibody is useful for Immunocytochemistry/Immunofluorescence, Western Blot and Immunohistochemistry. In Western Blot a band is seen ~52-64 kDa in SF9 cell lysate. There is no band in striatal tissue lysate. Do NOT boil samples.
Publications
Read Publication using
NB110-60019 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Rat. Reactivity with mouse has had mixed results. It does not react with human protein.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Dopamine D5R/DRD5 Antibody (SG4-D1b)

  • D(1B) dopamine receptor
  • D(5) dopamine receptor
  • D1beta dopamine receptor
  • DBDR
  • Dopamine D5 R
  • Dopamine D5 receptor
  • Dopamine D5R
  • dopamine receptor D1B
  • dopamine receptor D5
  • DRD1B
  • DRD1BDBDR
  • DRD1L2
  • DRD1L2MGC10601
  • DRD5

Background

Dopamine Receptor D5 (DRD5 or D5R/D5 receptor) belongs to GPCR1 family and represents dopamine receptor whose activity is mediated by G proteins that activate adenylyl cyclase. Localized in cell membranes as multi-pass trans-membrane protein, DRD5 exhibits widespread expression in CNS tissues- amygdala, frontal cortex, hippocampus, striatum, basal forebrain, hypothalamus, cerebellum and thalamus - whereas, at cellular level, the large aspiny neurons of neostriatum, which are typically cholinergic interneurons, only express DRD5. Besides stimulating adenyl cyclase activity, DRD5 also interacts with GABRG2 suggesting that it may modulate GABAAR-mediated activity via second messenger cascades as well as direct receptor-receptor interaction. Mice lacking DRD5 are less active in baseline locomotor exploration while their exploratory activity increases, suggesting its inhibitory effect on locomotion. DRD5 has been suggested to be implicated in regulating hypothalamic function and some forms of motor control.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Dopamine D5R/DRD5 Antibody (NB110-60019)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 2 applications: IHC, WB.


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WB
(1)
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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DRD5