Dopa Decarboxylase/DDC Antibody (413911) [Unconjugated]

Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/mL of Human Dopa Decarboxylase/DDC Monoclonal Antibody (Catalog # MAB3564) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Dopa Decarboxylase/DDC at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of Jurkat human acute T cell leukemia parental cell line and Dopa Decarboxylase/DDC Jurkat cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Dopa Decarboxylase/DDC Monoclonal Antibody (Catalog # MAB3564) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Dopa Decarboxylase/DDC at approximately 54 kDa (as indicated) in the parental Jurkat cell line, but is not detectable in knockout Jurkat cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, KO
• Clone: 413911
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Dopa Decarboxylase/DDC Antibody (413911) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human DDC
  Met1-Glu480
  Accession # P20711
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: DDC
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dopa Decarboxylase/DDC Antibody (413911) [Unconjugated]

• AADC
• AADCaromatic-L-amino-acid decarboxylase
• DDC
• dopa decarboxylase (aromatic L-amino acid decarboxylase)
• Dopa Decarboxylase
• EC 4.1.1
• EC 4.1.1.28

Background

Dopa decarboxylase (DDC), also known as aromatic amino acid decarboxylase, is a group II decarboxylase (1). The enzyme catalyzes the decarboxylation of aromatic L-amino acids to produce the corresponding amines. DDC produces the neurotransmitters dopamine and serotonin from L-Dopa and L-5-hydroxytryptophan, respectively. The inhibition of DDC could be used for the treatment of schizophrenia and Parkinson's disease (2).

1. Sandmeier, E. et al. (1994) Eur. J. Biochem. 221:997.
2. Bertoldi, M. et al. (1996) J. Biol. Chem. 271:23954.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DDC
• Uniprot:
  • P20711()