DNASE1L3 Antibody - BSA Free

Western Blot: DNASE1L3 Antibody [NBP2-16198] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DNase IL3 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: DNASE1L3 Antibody [NBP2-16198] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with DNase IL3 antibody [N3C3] diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/ Immunofluorescence: DNASE1L3 Antibody [NBP2-16198] - DNASE1L3 antibody [N3C3] detects DNASE1L3 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DNASE1L3 stained by DNASE1L3 antibody [N3C3] (NBP2-16198) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.

Western Blot: DNASE1L3 Antibody [NBP2-16198] -Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DNase IL3 antibody [N3C3] diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DNASE1L3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DNASE1L3 Antibody - BSA Free (NBP2-16198) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human DNASE1L3. The exact sequence is proprietary.
• Predicted Species: Rat (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DNASE1L3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for DNASE1L3 Antibody - BSA Free

• deoxyribonuclease gamma
• deoxyribonuclease I-like 3Liver and spleen DNase
• deoxyribonuclease I-like III
• DNAS1L3LSDDHP2
• DNase gamma
• DNase I homolog protein 2
• DNase I homolog protein DHP2
• DNase I-like 3
• EC 3.1.21.-
• EC 3.1.21.1
• LS-DNase

Background

This gene encodes a member of the DNase family. The protein hydrolyzes DNA, is not inhibited by actin, and mediates the breakdown of DNA during apoptosis. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: DNASE1L3
• Uniprot:
  • Q13609(Human)