DNAJC3 Antibody

Western Blot: DNAJC3 Antibody [NBP2-16195] - Sample (30 ug of whole cell lysate) A: Raji 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: DNAJC3 Antibody [NBP2-16195] - A431 cells were fixed in iced-cold MeOH for 5 min. Green: DNAJC3 protein stained by DNAJC3 antibody diluted at 1:500. Blue: Hoechst 33343 staining.

Immunohistochemistry-Paraffin: DNAJC3 Antibody [NBP2-16195] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:250 dilution.

Product Discontinued

DNAJC3 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human DNAJC3. The exact sequence is proprietary.
• Localization: Endoplasmic reticulum
• Marker: ER Stress Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DNAJC3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for DNAJC3 Antibody

• DnaJ (Hsp40) homolog, subfamily C, member 3
• HP58
• Interferon-induced, double-stranded RNA-activated protein kinase inhibitor
• P58FLJ21288
• P58IPKProtein kinase inhibitor p58
• PRKRIdnaJ homolog subfamily C member 3
• Protein kinase inhibitor of 58 kDa
• protein-kinase, interferon-inducible double stranded RNA dependent inhibitor

Background

The protein encoded by this gene contains multiple tetratricopeptide repeat (TPR) motifs as well as the highly conserved J domain found in DNAJ chaperone family members. It is a member of the tetratricopeptide repeat family of proteins and acts as an inhibitor of the interferon-induced, dsRNA-activated protein kinase (PKR). [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: DNAJC3
• Uniprot:
  • Q13217(Human)