Recombinant Human DNAJB8 His Protein

SDS-Page: Recombinant Human DNAJB8 Protein [NBP1-72504] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.25 mg/ml

Recombinant Human DNAJB8 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-232 of Human DNAJB8
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MANYYEVLGV QASASPEDIK KAYRKLALRW HPDKNPDNKE EAEKKFKLVS EAYEVLSDSK KRSLYDRAGC DSWRAGGGAS TPYHSPFDTG YTFRNPEDIF REFFGGLDPF SFEFWDSPFN SDRGGRGHGL RGAFSAGFGE FPAFMEAFSS FNMLGCSGGS HTTFSSTSFG GSSSGSSGFK SVMSSTEMIN GHKVTTKRIV ENGQERVEVE EDGQLKSVTV NGKEQLKWMD SK

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: DNAJB8
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 27.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 40% glycerol, 0.15 M NaCl, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human DNAJB8 His Protein

• DJ6
• DnaJ (Hsp40) homolog, subfamily B, member 8
• MGC33884
• subfamily B, member 8

Background

DNAJB8, also known as dnaJ homolog subfamily B member 8, is superior suppressors of aggregation and toxicity of disease-associated polyglutamine proteins. DNAJB8 is (de)acetylated at two conserved C-terminal lysines that are not involved in substrate binding, but do play a role in suppressing protein aggregation. Recombinant human DNAJB8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: DNAJB8