DNA polymerase theta Antibody

• Reactivity: Hu
• Applications: ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DNA polymerase theta Antibody Summary

• Description: Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.
• Immunogen: Peptide with sequence DTHQQLTCSNDES corresponding to internal region according to NP_955452.3.
• Epitope: internal region
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: POLQ
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:1000
• Application Notes: WB: Preliminary experiments in human brain (cerebellum lysates) and in lysates of HeLa gave no specific signal but low background.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DNA polymerase theta Antibody

• DKFZp781A0112
• DNA polymerase eta
• EC 2.7.7.7
• POLHDNA polymerase theta
• polymerase (DNA directed), theta
• PRO0327

Background

DNA polymerase theta, also referred to as POLQ, is highly expressed in the testes. POLQ has a 2,590 amino acid long isoform that is approximately 290kDa and a shorter 1,786 amino acid long isoform that is approximately 198kDa. POLQ is highly expressed in the testes, plays a role in DNA polymerase activity, and may be implicated in interstrand crosslink repair (ICR). Research on DNA polymerase theta has shown a connection to several diseases and disorders including stomach cancer, malaria, breast cancer, tuberculosis, and meningioma. POLQ has also been shown to have interactions with AP2S1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DNA polymerase theta Antibody (NBP1-36950)(1)

Publication using NBP1-36950

• Kawamura K, Bahar R, Seimiya M et al. DNA polymerase theta is preferentially expressed in lymphoid tissues and upregulated in human cancers. Int J Cancer 2004-03-01 [PMID: 14735462]

Bioinformatics

• Gene Symbol: POLQ
• Entrez:
  • 10721(Human)
• Uniprot:
  • NP_955452.3(Human)