DNA Polymerase epsilon subunit 3 Antibody

Images

 
Western Blot: DNA Polymerase epsilon subunit 3 Antibody [NB100-60438] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. NB100-60438 used for WB at 0.4 ...read more
Immunohistochemistry: DNA Polymerase epsilon subunit 3 Antibody [NB100-60438] - Sample: FFPE section of human ovarian carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- PolE3/p17 used ...read more
Immunohistochemistry-Paraffin: DNA Polymerase epsilon subunit 3 Antibody [NB100-60438] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-PolE3/p17 1:100. Detection: DAB staining using ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

DNA Polymerase epsilon subunit 3 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 97 and 147 of human DNA polymerase epsilon subunit 3 using the numbering given in entry NP_059139.2 (GeneID 54107).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLE3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 2-5 ug/mg
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DNA Polymerase epsilon subunit 3 Antibody

  • Arsenic-transactivated protein
  • asTP
  • CHARAC17arsenic transactivated protein
  • CHRAC-17
  • CHRAC17Ybl1
  • Chromatin accessibility complex 17 kDa protein
  • DNA polymerase epsilon p17 subunit
  • DNA polymerase epsilon subunit 3
  • DNA polymerase epsilon subunit p17
  • DNA polymerase II subunit 3
  • EC 2.7.7.7
  • histone fold protein CHRAC17
  • huCHRAC17
  • p17
  • polymerase (DNA directed), epsilon 3 (p17 subunit)
  • YBL1

Background

POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol POLE3
Uniprot