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DNA Polymerase epsilon subunit 3 Antibody

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Western Blot: DNA Polymerase epsilon subunit 3 Antibody [NB100-60438] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. NB100-60438 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.
Western Blot: DNA Polymerase epsilon subunit 3 Antibody [NB100-60438] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. NB100-60438 used for WB at 0.4 ...read more
Immunohistochemistry-Paraffin: DNA Polymerase epsilon subunit 3 Antibody [NB100-60438] - Section of human ovarian carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- PolE3/p17 used at a ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, PmSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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DNA Polymerase epsilon subunit 3 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 97 and 147 of human DNA polymerase epsilon subunit 3 using the numbering given in entry NP_059139.2 (GeneID 54107).
Predicted Species
Rat (100%), Primate (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLE3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 2 - 5 ug/mg lysate
  • Western Blot 1:2000- 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Reactivity Notes

Orangutan (100%).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DNA Polymerase epsilon subunit 3 Antibody

  • Arsenic-transactivated protein
  • asTP
  • CHARAC17arsenic transactivated protein
  • CHRAC-17
  • CHRAC17Ybl1
  • Chromatin accessibility complex 17 kDa protein
  • DNA polymerase epsilon p17 subunit
  • DNA polymerase epsilon subunit 3
  • DNA polymerase epsilon subunit p17
  • DNA polymerase II subunit 3
  • EC 2.7.7.7
  • histone fold protein CHRAC17
  • huCHRAC17
  • p17
  • polymerase (DNA directed), epsilon 3 (p17 subunit)
  • YBL1

Background

POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: TPPP/p25 Antibody [NBP2-34031] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral cortex tissue.
Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP2-34031] - Staining of human cerebral cortex, liver, lower gastrointestinal and pancreas using Anti-TPPP antibody NBP2-34031 (A) shows similar protein distribution across tissues to independent antibody NBP1-80962 (B).
Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP2-34031] - Analysis in human cerebral cortex and liver tissues using NBP2-34031 antibody. Corresponding TPPP RNA-seq data are presented for the same tissues.
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Immunocytochemistry/Immunofluorescence: Calcium-binding-protein-P22 Antibody [NBP1-33736] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calcium binding protein P22 protein stained by Calcium binding protein P22 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Calcium-binding-protein-P22 Antibody [NBP1-33736] - Paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.
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Immunohistochemistry-Paraffin: NRSN1 Antibody [NBP2-31844] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunocytochemistry/Immunofluorescence: NRSN1 Antibody [NBP2-31844] - Immunofluorescent staining of human cell line Hep G2 shows localization to vesicles.
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Immunohistochemistry-Paraffin: TMED2 Antibody [NBP1-80963] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Western Blot: TMED2 Antibody [NBP1-80963] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/Immunofluorescence: TMED2 Antibody [NBP1-80963] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
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Flow Cytometry: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - An intracellular stain was performed on HeLa cells with Caspase-3 Antibody (31A1067) - (Pro and Active) NB100-56708 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).
Flow Cytometry: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - An intracellular stain was performed on NIH3T3 cells with Caspase-3 Antibody (31A1067) - (Pro and Active) Antibody NB100-56708AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Paclitaxel in combination with MWE retarded tumor growth in a human bladder carcinoma TSGH 8301 xenograft model. The levels of total (t-PTEN) and phospho-PTEN (p-PTEN) and Caspase 3 in the tumor specimens were determined by Western blotting and then quantified using beta-actin as the protein loading control; the results are expressed as a percentage of the control. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep20417), licensed under a CC-BY licence.
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Immunohistochemistry-Paraffin: SUB1 Antibody [NBP1-82454] - Staining of human rectum shows strong nuclear positivity in glandular cells.
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Immunohistochemistry-Paraffin: RRP4 Antibody [NBP2-97759] - Immunochemical staining of human RRP4 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
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Immunocytochemistry/Immunofluorescence: MRPL28 Antibody (3B9F2) [NBP3-15267] - Immunofluorescence analysis of PC-12 cells using MRPL28 Rabbit mAb (NBP3-15267) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation: MRPL28 Antibody (3B9F2) [NBP3-15267] - Immunoprecipitation analysis of 300ug extracts of K-562 cells using 3ug MRPL28 antibody (NBP3-15267). Western blot was performed from the immunoprecipitate using MRPL28 antibody (NBP3-15267) at a dilition of 1:1000.
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Immunohistochemistry-Frozen: CD4 Antibody [NBP1-19371] - Staining in mouse liver tissue.
Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Alcohol-fixed paraffin-embedded rat spleen tissue. Dilution 1:150. Image from verified customer review.
Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on Jurkat cells with CD4 Antibody NBP1-19371R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.
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Bioinformatics

Gene Symbol POLE3
Uniprot