DNA Polymerase epsilon subunit 3 Antibody

Western Blot: DNA Polymerase epsilon subunit 3 Antibody [NB100-60438] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. NB100-60438 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.

Immunohistochemistry-Paraffin: DNA Polymerase epsilon subunit 3 Antibody [NB100-60438] - Section of human ovarian carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- PolE3/p17 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB

• Reactivity: Hu, Mu, Rt, Bv, Pm
• Applications: WB, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

DNA Polymerase epsilon subunit 3 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 97 and 147 of human DNA polymerase epsilon subunit 3 using the numbering given in entry NP_059139.2 (GeneID 54107).
• Predicted Species: Rat (100%), Primate (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLE3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 2 - 5 ug/mg lysate
  • Western Blot 1:2000- 1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Reactivity Notes

Orangutan (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DNA Polymerase epsilon subunit 3 Antibody

• Arsenic-transactivated protein
• asTP
• CHARAC17arsenic transactivated protein
• CHRAC-17
• CHRAC17Ybl1
• Chromatin accessibility complex 17 kDa protein
• DNA polymerase epsilon p17 subunit
• DNA polymerase epsilon subunit 3
• DNA polymerase epsilon subunit p17
• DNA polymerase II subunit 3
• EC 2.7.7.7
• histone fold protein CHRAC17
• huCHRAC17
• p17
• polymerase (DNA directed), epsilon 3 (p17 subunit)
• YBL1

Background

POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLE3
• Uniprot:
  • NP_059139.2(Human)