DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1) [DyLight 405]

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Product Details

Summary
Reactivity Hu, Mu, Md, Pm, Dr(-)Species Glossary
Applications WB, ICC/IF
Clone
3C5.1
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 405

DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1) [DyLight 405] Summary

Immunogen
Human DNA Polymerase epsilon purified from HeLa cells.
Localization
Nuclear
Specificity
This is specific to the human DNA pol epsilon and does not recognize DNA pol alpha, beta or delta.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
POLE
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
This DNA Polymerase epsilon (3C5.1) antibody can be used in Western blot, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In WB, it detects a single band of 220 kDa (in HeLa extracts) representing the catalytic subunit of DNA Polymerase epsilon. NB100-115 will immunoprecipitate, but only in the absence of salt and even then poorly.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mink samples. This antibody does not work on Drosophilia or the baculovirus host cell, and weakly, if at all, on S. cerivisae POLE.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1) [DyLight 405]

  • DKFZp434F222
  • DNA polymerase epsilon catalytic subunit A
  • DNA polymerase II subunit A
  • EC 2.7.7
  • EC 2.7.7.7
  • POLE1FLJ21434
  • polymerase (DNA directed), epsilon

Background

DNA Polymerase epsilon, also called DNA polymerase epsilon subunit 3 (POLE3) is a histone-fold protein which interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. DNA Polymerase epsilon generates a complex with DNA polymerase epsilon subunit CHRAC1 and associates with naked DNA, which is then incorporated into chromatin, facilitated through the nucleosome-remodeling activity of ISWI/SNF2H and ACF1. DNA Polymerase epsilon is a component of the epsilon DNA polymerase complex consisting of four subunits: POLE, POLE2, POLE3 and POLE4, wherein, the interaction with POLE4 is a prerequisite for further binding with POLE or POLE2. DNA Polymerase epsilon interacts with CHRAC1, and together with CHRAC1, ACF1 and ISWI/SNF2H proteins, it forms the ISWI chromatin-remodeling complex, CHRA. DNA Polymerase epsilon histone-fold protein dimers combine within larger enzymatic complexes for facilitating the processes of DNA packaging, replication, and transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NB100-115B
DyLight 350 Labeled NB100-115UV
DyLight 488 Labeled NB100-115G
DyLight 550 Labeled NB100-115R
DyLight 650 Labeled NB100-115C
DyLight 680 Labeled NB100-115FR
DyLight 755 Labeled NB100-115IR
FITC Labeled NB100-115F
HRP Labeled NB100-115H

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Bioinformatics

Gene Symbol POLE