DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1) [HRP]

• Reactivity: Hu, Mu, Md, Pm, Dr(-)
• Applications: WB
• Clone: 3C5.1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: HRP

DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1) [HRP] Summary

• Immunogen: Human DNA Polymerase epsilon purified from HeLa cells.
• Localization: Nuclear
• Specificity: This is specific to the human DNA pol epsilon and does not recognize DNA pol alpha, beta or delta.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: POLE
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This DNA Polymerase epsilon (3C5.1) antibody can be used in Western blot, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In WB, it detects a single band of 220 kDa (in HeLa extracts) representing the catalytic subunit of DNA Polymerase epsilon. NB100-115 will immunoprecipitate, but only in the absence of salt and even then poorly. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Reactivity Notes

Mink samples. This antibody does not work on Drosophilia or the baculovirus host cell, and weakly, if at all, on S. cerivisae POLE.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1) [HRP]

• DKFZp434F222
• DNA polymerase epsilon catalytic subunit A
• DNA polymerase II subunit A
• EC 2.7.7
• EC 2.7.7.7
• POLE1FLJ21434
• polymerase (DNA directed), epsilon

Background

DNA polymerase epsilon from HeLa cells differs from yeast pol epsilon in that HeLa pol epsilon contains two subunits of 220 and 55 kDa, whereas yeast pol epsilon has one large subunit and three smaller subunits. Previous studies of template specificity of HeLa pol epsilon, which appears to be required for long patch DNA repair in permeabilized cells, indicate pol epsilon prefers long stretches of single-stranded regions in poly(dA)-oligo(dT) as template. HeLa pol alpha and pol delta prefer activated DNA and alternating poly(dA-dT) as templates, respectively. Pol epsilon retains its processivity in the absence of proliferating cell nuclear antigen (PCNA), unlike pol delta. PCNA can also interact with Pol epsilon on linear DNA templates, even in the absence of other auxiliary factors (replication factor C, replication factor A), and thereby stimulate its primer recognition and DNA synthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLE