DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1) [Alexa Fluor® 488]

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Product Details

Summary
Reactivity Hu, Mu, Md, Pm, Dr(-)Species Glossary
Applications WB, ICC/IF
Clone
3C5.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 488

DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1) [Alexa Fluor® 488] Summary

Immunogen
Human DNA Polymerase epsilon purified from HeLa cells.
Localization
Nuclear
Specificity
This is specific to the human DNA pol epsilon and does not recognize DNA pol alpha, beta or delta.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
POLE
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
This DNA Polymerase epsilon (3C5.1) antibody can be used in Western blot, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In WB, it detects a single band of 220 kDa (in HeLa extracts) representing the catalytic subunit of DNA Polymerase epsilon. NB100-115 will immunoprecipitate, but only in the absence of salt and even then poorly.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mink samples. This antibody does not work on Drosophilia or the baculovirus host cell, and weakly, if at all, on S. cerivisae POLE.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1) [Alexa Fluor® 488]

  • DKFZp434F222
  • DNA polymerase epsilon catalytic subunit A
  • DNA polymerase II subunit A
  • EC 2.7.7
  • EC 2.7.7.7
  • POLE1FLJ21434
  • polymerase (DNA directed), epsilon

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 647 Labeled NB100-115AF647
Alexa Fluor 700 Labeled NB100-115AF700

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Bioinformatics

Gene Symbol POLE