Western Blot: DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1) [NB100-115] - SDS-polyacrylamide gel analysis of HeLa DNA pol purified by Mono S FPLC. The Mono S fraction (0.1 mg) was electrophoresed on a 7% ...read more
Immunohistochemistry-Paraffin: DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1) [NB100-115] - Analysis of a FFPE tissue section of human kidney using 1:200 dilution of DNA Polymerase epsilon catalytic subunit ...read more
This DNA Polymerase epsilon (3C5.1) antibody can be used in Western blot, Immunohistochemistry and Immunoprecipitation. In WB, it detects a single band of 220 kDa (in HeLa extracts) representing the catalytic subunit of DNA Polymerase epsilon. NB100-115 will immunoprecipitate, but only in the absence of salt and even then poorly. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunohistochemistry reported in scientific literature (PMID: 29935943).
220 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Mink samples. This antibody does not work on Drosophilia or the baculovirus host cell, and weakly, if at all, on S. cerivisae POLE.
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1)
DNA polymerase epsilon catalytic subunit A
DNA polymerase II subunit A
polymerase (DNA directed), epsilon
DNA Polymerase epsilon, also called DNA polymerase epsilon subunit 3 (POLE3) is a histone-fold protein which interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. DNA Polymerase epsilon generates a complex with DNA polymerase epsilon subunit CHRAC1 and associates with naked DNA, which is then incorporated into chromatin, facilitated through the nucleosome-remodeling activity of ISWI/SNF2H and ACF1. DNA Polymerase epsilon is a component of the epsilon DNA polymerase complex consisting of four subunits: POLE, POLE2, POLE3 and POLE4, wherein, the interaction with POLE4 is a prerequisite for further binding with POLE or POLE2. DNA Polymerase epsilon interacts with CHRAC1, and together with CHRAC1, ACF1 and ISWI/SNF2H proteins, it forms the ISWI chromatin-remodeling complex, CHRA. DNA Polymerase epsilon histone-fold protein dimers combine within larger enzymatic complexes for facilitating the processes of DNA packaging, replication, and transcription.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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