DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1)

Images

 
Western Blot: DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1) [NB100-115] - SDS-polyacrylamide gel analysis of HeLa DNA pol purified by Mono S FPLC. The Mono S fraction (0.1 mg) was electrophoresed on a 7% ...read more
Immunohistochemistry-Paraffin: DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1) [NB100-115] - Analysis of a FFPE tissue section of human kidney using 1:200 dilution of DNA Polymerase epsilon catalytic subunit ...read more

Product Details

Summary
Reactivity Hu, Mu, Md, Pm, Dr(-)Species Glossary
Applications WB, IHC, IHC-P, IP
Clone
3C5.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1) Summary

Immunogen
Human DNA Polymerase epsilon purified from HeLa cells.
Localization
Nuclear
Specificity
This is specific to the human DNA pol epsilon and does not recognize DNA pol alpha, beta or delta.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
POLE
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 5 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500
Application Notes
This DNA Polymerase epsilon (3C5.1) antibody can be used in Western blot, Immunohistochemistry and Immunoprecipitation. In WB, it detects a single band of 220 kDa (in HeLa extracts) representing the catalytic subunit of DNA Polymerase epsilon. NB100-115 will immunoprecipitate, but only in the absence of salt and even then poorly. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunohistochemistry reported in scientific literature (PMID: 29935943).
Theoretical MW
220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-115 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Mink samples. This antibody does not work on Drosophilia or the baculovirus host cell, and weakly, if at all, on S. cerivisae POLE.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1)

  • DKFZp434F222
  • DNA polymerase epsilon catalytic subunit A
  • DNA polymerase II subunit A
  • EC 2.7.7
  • EC 2.7.7.7
  • POLE1FLJ21434
  • polymerase (DNA directed), epsilon

Background

DNA Polymerase epsilon, also called DNA polymerase epsilon subunit 3 (POLE3) is a histone-fold protein which interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. DNA Polymerase epsilon generates a complex with DNA polymerase epsilon subunit CHRAC1 and associates with naked DNA, which is then incorporated into chromatin, facilitated through the nucleosome-remodeling activity of ISWI/SNF2H and ACF1. DNA Polymerase epsilon is a component of the epsilon DNA polymerase complex consisting of four subunits: POLE, POLE2, POLE3 and POLE4, wherein, the interaction with POLE4 is a prerequisite for further binding with POLE or POLE2. DNA Polymerase epsilon interacts with CHRAC1, and together with CHRAC1, ACF1 and ISWI/SNF2H proteins, it forms the ISWI chromatin-remodeling complex, CHRA. DNA Polymerase epsilon histone-fold protein dimers combine within larger enzymatic complexes for facilitating the processes of DNA packaging, replication, and transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DNA Polymerase epsilon catalytic subunit A Antibody (NB100-115)(9)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IHC, WB.


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IHC
(1)
WB
(1)
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(2)
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Showing Publications 1 - 9 of 9.
Publications using NB100-115 Applications Species
Gaspar J M, Mendes N F et al. Downregulation of HIF complex in the hypothalamus exacerbates diet-induced obesity. Brain Behav Immun Jan 10 2018 [PMID: 29935943] (IHC, Mouse) IHC Mouse
Asahara H, Li Y, Fuss J, Haines DS, Vlatkovic N, Boyd MT, Linn S. Stimulation of human DNA polymerase epsilon by MDM2. Nucleic Acids Res;31(9):2451-9. 2003 May 1. [PMID: 12711691]
Liu W, Linn S. Proteolysis of the human DNA polymerase epsilon catalytic subunit by caspase-3 and calpain specifically during apoptosis. Nucleic Acids Res;28(21):4180-8. 2000 Nov 1. [PMID: 11058115]
Vlatkovic N, Guerrera S, Li Y, Linn S, Haines DS, Boyd MT. MDM2 interacts with the C-terminus of the catalytic subunit of DNA polymerase epsilon. Nucleic Acids Res;28(18):3581-6. 2000 Sep 15. [PMID: 10982879]
Li Y, Pursell ZF, Linn S. Identification and cloning of two histone fold motif-containing subunits of HeLa DNA polymerase epsilon. J Biol Chem;275(30):23247-52. 2000 Jul 28. [PMID: 10801849]
Kramata P, Downey KM, Paborsky LR. Incorporation and excision of 9-(2-phosphonylmethoxyethyl)guanine (PMEG) by DNA polymerase delta and epsilon in vitro. J Biol Chem;273(34):21966-71. 1998 Aug 21. [PMID: 9705337]
Islas L, Fairley CF, Morgan WF. DNA synthesis on discontinuous templates by human DNA polymerases: implications for non-homologous DNA recombination. Nucleic Acids Res;26(16):3729-38. 1998 Aug 15. [PMID: 9685489]
Li Y, Asahara H, Patel VS, Zhou S, Linn S. Purification, cDNA cloning, and gene mapping of the small subunit of human DNA polymerase epsilon. J Biol Chem;272(51):32337-44. 1997 Dec 19. [PMID: 9405441]
Chui, G, Linn, S. Further characterization of HeLa DNA polymerase epsilon. J Biol Chem;270(14):7799-808. 1995 Apr 7. [PMID: 7536191] (WB, Mouse) WB Mouse

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-115AF350-0.1ml
Alexa Fluor 405 NB100-115AF405
Alexa Fluor 488 NB100-115AF488
Alexa Fluor 532 NB100-115AF532-0.1ml
Alexa Fluor 594 NB100-115AF594-0.1ml
Alexa Fluor 647 NB100-115AF647
Alexa Fluor 700 NB100-115AF700
Alexa Fluor 750 NB100-115AF750-0.1ml
Biotin NB100-115B
DyLight 350 NB100-115UV
DyLight 488 NB100-115G
DyLight 550 NB100-115R
DyLight 594 NB100-115DL594-0.1ml
DyLight 650 NB100-115C
DyLight 680 NB100-115FR
DyLight 680 NB100-115FR
DyLight 755 NB100-115IR
FITC NB100-115F
HRP NB100-115H
Janelia Fluor 549 NB100-115JF549-0.1ml
Janelia Fluor 646 NB100-115JF646-0.1ml

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Bioinformatics

Gene Symbol POLE