DMP-1 Antibody (560722) [Biotin]

• Reactivity: Hu
• Applications: ELISA(Det)
• Clone: 560722
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin
• Concentration: LYOPH

DMP-1 Antibody (560722) [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human DMP-1 isoform 2
  Leu17-Tyr497
  Accession # NP_001073380
• Specificity: Detects human DMP-1 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant mouse DMP-1, recombinant human (rh) CD44, and rhComplement Factor H is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DMP1
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Capture: Human DMP-1 Antibody (Catalog # MAB4129)
  ELISA Detection: Human DMP-1 Biotinylated Antibody (Catalog # BAM41291)
  Standard: Recombinant Human DMP-1 (Catalog # 4129-DM)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DMP-1 Antibody (560722) [Biotin]

• ARHP
• ARHR
• dentin matrix acidic phosphoprotein 1
• dentin matrix acidic phosphoprotein
• Dentin matrix protein 1
• DMP1
• DMP-1

Background

Dentin matrix protein 1 (DMP-1) is a member of the SIBLING family that also includes bone sialoprotein, dentin sialophosphoprotein, MEPE, and osteopontin. These highly phosphorylated integrin-binding proteins are rich in acidic amino acids and function in the formation of calcified bone and tooth matrix (1, 2). The phosphate content, spacing of acidic residues, and calcium-dependent dimerization of DMP-1 contribute to its ability to sequester calcium phosphate clusters and promote hydroxyapatite (HA) crystal formation (3-5). Rodent DMP-1 is cleaved by BMP-1 family proteases at a single site which is conserved in human, generating a 37 kDa N-terminal and a 57 kDa C-terminal fragment (6). The N-terminal fragment in rat carries chondroitin sulfate (7). The C-terminal fragment alone can nucleate HA crystals, while crystal growth into a needle-like morphology is inhibited by the N-terminal fragment (3, 4). Crystal maturation is dependent on the presence of type I collagen (4). DMP-1 is required for odontoblast differentiation as well as dentin formation (8). Nonphosphorylated DMP-1 is targeted to the nucleus, where it activates the transcription of odontoblast and osteoblast specific genes (9, 10). Early in osteoblast maturation, nuclear DMP-1 is extensively phosphorylated by casein kinase II, triggering its secretion (9). DMP-1 mutations in humans are associated with hypophosphatemia and FGF23 overexpression (11, 12). DMP-1 induces the activation of proMMP-9 and displaces mature MMP-9 from TIMP1 (13). DMP-1 tethering of MMP-9 to the cell surface via CD44 and integrins alpha v beta 3 and alpha v beta 5 promotes tumor cell invasiveness in vitro (14). Full length DMP-1 circulates in human serum in a tight complex with complement factor H (13, 14). When first bound to CD44 or integrin alpha v beta 3, DMP-1 can anchor factor H to the cell surface and protect the cell from complement-mediated lysis (15). Mature human DMP-1 shares 61%‑67% amino acid sequence identity with bovine, mouse, and rat DMP-1.

1. Qin, C. et al. (2004) Crit. Rev. Oral Biol. Med. 15:126.
2. Hirst, K.L. et al. (1997) Genomics 42:38.
3. He, G. et al. (2003) Nat. Mater. 2:552.
4. Gajjeraman, S. et al. (2007) J. Biol. Chem. 282:1193.
5. He, G. et al. (2005) Biochemistry 44:16140.
6. Steiglitz, B.M. et al. (2004) J. Biol. Chem. 279:980.
7. Qin, C. et al. (2006 J. Biol. Chem. 281:8034.
8. Lu, Y. et al. (2007) Dev. Biol. 303:191.
9. Narayanan, K. et al. (2003) J. Biol. Chem. 278:17500.
10. Narayanan, K. et al. (2006) J. Biol. Chem. 281:19064.
11. Lorenz-Depiereux, B. et al. (2006) Nat. Genet. 38:1248.
12. Feng, J.Q. et al. (2006) Nat. Genet. 38:1310.
13. Fedarko, N.S. et al. (2004) FASEB J. 18:735.
14. Karadag, A. et al. (2005) Cancer Res. 65:11545.
15. Jain, A. et al. (2002) J. Biol. Chem. 277:13700.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DMP1
• Uniprot:
  • NP_001073380()