DLX5 Overexpression Lysate

Images

 
Western Blot: Dlx5 Overexpression Lysate (Adult Normal) [NBL1-09921] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Dlx5.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

DLX5 Overexpression Lysate Summary

Description

DLX5 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC202525

Accession#: NM_005221

Protein Tag: C-MYC/DDK
Gene
DLX5

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
31.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for DLX5 Overexpression Lysate

  • distal-less homeo box 5
  • distal-less homeobox 5
  • DLX5
  • homeobox protein DLX-5

Background

This gene encodes a member of a homeobox transcription factor gene family similiar to the Drosophila distal-less gene. The encoded protein may play a role in bone development and fracture healing. Mutation in this gene, which is located in a tail-to-tail configuration with another member of the family on the long arm of chromosome 7, may be associated with split-hand/split-foot malformation. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

355-BM/CF
Species: Hu, Mu, Rt
Applications: BA
NBP1-85929
Species: Hu
Applications: ChIP, IHC, IHC-P, WB
MAB7547
Species: Hu
Applications: ICC, WB
NBP1-85445
Species: Hu
Applications: IHC, IHC-P, WB
MAB1419
Species: Hu, Rt
Applications: CyTOF-ready, ICC, IHC, ICFlow
AF4014
Species: Hu
Applications: WB
H00001746-M01
Species: Hu, Mu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
314-BP/CF
Species: Hu
Applications: BA, BA
DPI00
Species: Hu
Applications: ELISA
NB100-56326
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
DCC270
Species: Hu
Applications: ELISA
NBP1-89133
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NB110-3638
Species: Hu, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, RI, WB
NBP1-89131
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-32263
Species: Hu, Mu
Applications: IHC, IP, WB
NBP1-89134
Species: Hu
Applications: IHC, IHC-P
355-BM/CF
Species: Hu, Mu, Rt
Applications: BA
H00001745-M01
Species: Hu
Applications: ELISA, ICC/IF, IP, S-ELISA, WB
NBL1-09921
Species: Hu
Applications: WB

Publications for DLX5 Lysate (NBL1-09921) (0)

There are no publications for DLX5 Lysate (NBL1-09921).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for DLX5 Lysate (NBL1-09921) (0)

There are no reviews for DLX5 Lysate (NBL1-09921). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DLX5 Lysate (NBL1-09921) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional DLX5 Products

Bioinformatics Tool for DLX5 Lysate (NBL1-09921)

Discover related pathways, diseases and genes to DLX5 Lysate (NBL1-09921). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for DLX5 Lysate (NBL1-09921)

Discover more about diseases related to DLX5 Lysate (NBL1-09921).
 

Pathways for DLX5 Lysate (NBL1-09921)

View related products by pathway.

PTMs for DLX5 Lysate (NBL1-09921)

Learn more about PTMs related to DLX5 Lysate (NBL1-09921).
 

Research Areas for DLX5 Lysate (NBL1-09921)

Find related products by research area.

Blogs on DLX5

There are no specific blogs for DLX5, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our DLX5 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol DLX5