Human Dkk-4 ELISA Kit (Colorimetric) Summary
| Sensitivity |
10 pg/mL |
| Inter-Assay |
CV% < 12% |
| Intra-Assay |
CV% < 10% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
DKK4 |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
DKK4 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human DKK4. |
Reactivity Notes
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Assay Diluent (Item E): 5x concentrated buffer.
- Detection Antibody Dkk-4 (Item F): Biotinylated anti-human Dkk-4 (each vial is enoughto assay half microplate).
- Dkk-4 Microplate (Item A): 96 wells coated with anti-human Dkk-4.
- HRP-Streptavidin concentrates (Item G): 200x concentrated HRP-conjugatedStreptavidin.
- Standard Protein (Item C): Human Dkk-4. 1 vial is enough to rum each standard induplicate.
- Stop Solution (Item I): 0.2 M sulfuric acid.
- TMB One-Step Substrate Reagent (Item H): 3, 3’, 5, 5’-tetramethylbenzidine (TMB) inbuffered solution.
- Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human Dkk-4 ELISA Kit (Colorimetric)
Background
DKK4 is a secreted protein that is a member of the dickkopf family. Like some other members of the dickkopf family DKK4 contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The activity of DKK4 is modulated by binding to the Wnt co-receptor and the co-factor kremen 2, and it is proposed that it is also an inhibitor of the Wnt signaling pathway.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Dkk-4 ELISA Kit (KA1707). (Showing 1 - 1 of 1 FAQ).
-
wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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