Human Dkk-4 ELISA Kit (Colorimetric)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      KA1707
    • Availability
      Product Discontinued

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Human Dkk-4 ELISA Kit (Colorimetric) Summary

Sensitivity
10 pg/mL
Inter-Assay
CV% < 12%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
DKK4

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
DKK4 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human DKK4.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent (Item E): 5x concentrated buffer.
  2. Detection Antibody Dkk-4 (Item F): Biotinylated anti-human Dkk-4 (each vial is enoughto assay half microplate).
  3. Dkk-4 Microplate (Item A): 96 wells coated with anti-human Dkk-4.
  4. HRP-Streptavidin concentrates (Item G): 200x concentrated HRP-conjugatedStreptavidin.
  5. Standard Protein (Item C): Human Dkk-4. 1 vial is enough to rum each standard induplicate.
  6. Stop Solution (Item I): 0.2 M sulfuric acid.
  7. TMB One-Step Substrate Reagent (Item H): 3, 3’, 5, 5’-tetramethylbenzidine (TMB) inbuffered solution.
  8. Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human Dkk-4 ELISA Kit (Colorimetric)

  • dickkopf (Xenopus laevis) homolog 4
  • dickkopf homolog 4 (Xenopus laevis)
  • dickkopf-4
  • dickkopf-related protein 4
  • Dkk4
  • Dkk-4
  • hDkk-4
  • MGC129562
  • MGC129563

Background

DKK4 is a secreted protein that is a member of the dickkopf family. Like some other members of the dickkopf family DKK4 contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The activity of DKK4 is modulated by binding to the Wnt co-receptor and the co-factor kremen 2, and it is proposed that it is also an inhibitor of the Wnt signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Dkk-4 ELISA Kit (KA1707). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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Bioinformatics

Gene Symbol DKK4